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Chitotriosidase/CHIT1 Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Chitotriosidase/CHIT1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Chitotriosidase/CHIT1
Ala22-Ser464
Accession # Q9D7Q1
Specificity
Detects mouse Chitotriosidase/CHIT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human CHIT1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CHIT1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using AF5325.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chitotriosidase/CHIT1 Antibody

  • CHI3
  • CHIT1
  • CHITCHI3
  • chitinase 1 (chitotriosidase)
  • chitinase-1
  • Chitotriosidase
  • chitotriosidase-1
  • EC 3.2.1.14
  • FLJ00314
  • MGC125322
  • plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Background

Chitotriosidase, encoded by the mouse CHIT1 gene, is a typical member of the chitinase family (1). It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract (2). CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form (2‑4). Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease (5). The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme (ACE) (5). Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (6).

  1. Zheng, T. et al. (2005) Gene 357:37.
  2. Boot, R.G. et al. (2006) J. Histochem. Cytochem. 53:1283.
  3. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
  4. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
  5. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
  6. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chitotriosidase/CHIT1 Antibody (AF5325)(2)

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Bioinformatics

Gene Symbol CHIT1
Uniprot