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CHIP/STUB1 Antibody

Images

 
Western Blot: CHIP/STUB1 Antibody [NBP1-30936] - Analysis of antibody on 293T (lane A) and Hep G2 (lane B), diluted at 1:1000 with 10% SDS PAGE gel.
Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody [NBP1-30936] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP1-30936] - Paraffin-embedded mouse esophagus. STUB1 antibody [C1C3] diluted at 1:500.
Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP1-30936] - Paraffin-embedded ES2 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CHIP/STUB1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CHIP/STUB1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (100%), Chicken (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STUB1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for CHIP/STUB1 Antibody

  • Antigen NY-CO-7
  • Carboxy terminus of Hsp70-interacting protein
  • CHIP
  • CHIPSTIP1 homology and U box-containing protein 1
  • CLL-associated antigen KW-8
  • E3 ubiquitin-protein ligase CHIP
  • EC 6.3.2.-
  • heat shock protein A binding protein 2 (c-terminal)
  • HSPABP2
  • NY-CO-7
  • SDCCAG7
  • serologically defined colon cancer antigen 7
  • STIP1 homology and U-box containing protein 1
  • STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
  • STUB1
  • UBOX1

Background

STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STUB1