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CENPN Antibody

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Western Blot: CENPN Antibody [NBP1-79664] - Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate
Immunocytochemistry/ Immunofluorescence: CENPN Antibody [NBP1-79664] - Sample Type: MCF7, Primary Antibody Dilution: 4 ug/ml
Immunocytochemistry/ Immunofluorescence: CENPN Antibody [NBP1-79664] - Sample Type : DNA/ACA
Immunocytochemistry/ Immunofluorescence: CENPN Antibody [NBP1-79664] - HeLa Primary Antibody Dilution: 4 ug/ml Secondary Antibody : Anti-rabbit Alexa 546 Secondary Antibody Dilution: 2 ug/ml

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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CENPN Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptide directed towards the middle region of human CENPNThe immunogen for this antibody is CENPN. Peptide sequence SFKKILQRALKNVTVSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYS. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CENPN
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1.0 ug/ml
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-79664.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for CENPN Antibody

  • BM039
  • C16orf60
  • CENP-N
  • centromere protein N
  • chromosome 16 open reading frame 60
  • FLJ13607
  • FLJ22660
  • ICEN32
  • Interphase centromere complex protein 32

Background

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on whichthe transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by thepresence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPN is anadditional factor required for centromere assembly (Foltz et al., 2006 (PubMed 16622419)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CENPN Antibody (NBP1-79664)(1)

Reviews for CENPN Antibody (NBP1-79664) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CENPN Antibody (NBP1-79664) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CENPN
Uniprot