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CENPT Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CENPT Antibody [NBP2-34093] - Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: CENPT Antibody [NBP2-34093] - Staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CENPT Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PLHDGVEEAEKKMEEEGVSVSEMEATGAQGPSRVEEAEGHTEVTEAEGSQGTAEADGPGASSGDEDASGRAASPESASSTPESLQARRHHQFLEP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CENPT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CENPT Protein (NBP2-34093PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CENPT Antibody

  • C16orf56
  • CENP-Tchromosome 16 open reading frame 56
  • centromere protein T
  • FLJ13111
  • FLJ43376
  • ICEN22
  • Interphase centromere complex protein 22

Background

Centromere protein T (CENP-T) is a component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CENPT
Uniprot