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CDC45L Antibody (4C2)

Images

 
Western Blot: CDC45L Antibody (4C2) [H00008318-M02] - CDC45L monoclonal antibody (M02), clone 4C2 Analysis of CDC45L expression in Jurkat.
Sandwich ELISA: CDC45L Antibody (4C2) [H00008318-M02] - Detection limit for recombinant GST tagged CDC45L is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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CDC45L Antibody (4C2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CDC45L (NP_003495, 477 a.a. ~ 566 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS
Specificity
CDC45L - CDC45 cell division cycle 45-like (S. cerevisiae)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDC45
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Reviewed Applications
Read 1 Review rated 3
using
H00008318-M02 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CDC45L Antibody (4C2)

  • CDC45 cell division cycle 45-like (S. cerevisiae)
  • cell division cycle 45 homolog (S. cerevisiae)
  • cell division cycle 45-like 2
  • human CDC45
  • S.cerevisiae, homolog)-like

Background

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC45L Antibody (H00008318-M02) (0)

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Review for CDC45L Antibody (H00008318-M02) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00008318-M02:
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WB
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
WB Human 08/10/2010
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Summary

ApplicationWestern Blot
Sample TestedHCT116 chromatin-enriched fraction, Sample Amount: 50 ug
SpeciesHuman

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Secondary Antibodies

 

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Verified Customer
08/10/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDC45
Entrez
OMIM
Uniprot