![Immunohistochemistry-Paraffin: CD99 Antibody (SPM586) - Azide and BSA Free [NBP3-11457] - Human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).](http://images.novusbio.com/fullsize/nbp3-11457_mouse-monoclonal-cd99-antibody-spm586-azide-and-bsa-free-24120258211688.jpg "Immunohistochemistry-Paraffin: CD99 Antibody (SPM586) - Azide and BSA Free [NBP3-11457] - Human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | SPM586 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified by Protein L. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44693). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Purified E-rosette forming cells from human peripheral blood lymphocytes |
| Localization | Cell surface |
| Marker | Ewing's Sarcoma Marker |
| Specificity | Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This monoclonal antibody shows a very similar reactivity to other CD99 monoclonal antibodys (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD99 |
| Purity | Protein L purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. This antibody is CyTOF ready. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein L purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CD99 Antibody (NBP3-11457)Find related products by research area.
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| Gene Symbol | CD99 |
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