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CD9 Antibody (1021007) [Unconjugated]

Images

 
Western blot shows lysates of TIME human endothelial cell line and human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB25292) followed by HRP-conjugated ...read more
Human peripheral blood platelets were stained with A) Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB25292) or B) isotype control antibody (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated Secondary ...read more
CD9 was detected in immersion fixed RPMI 8226 human multiple myeloma cell line (left panel; positive staining) and U937 human histiocytic lymphoma cell line (right panel; negative staining) using Mouse Anti-Human CD9 ...read more
CD9 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB25292) at 5 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clone
1021007
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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CD9 Antibody (1021007) [Unconjugated] Summary

Immunogen
Chinese Hamster Ovary cell line, CHO-derived human CD9
Ser112-Ile195
Accession # P21926
Specificity
Detects human CD9 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB25292 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD9 Antibody (1021007) [Unconjugated]

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Background

CD9, also known as Tspan29, is a 24-27 kDa cell surface protein belonging to the tetraspanin family (1). Common to other tetraspanins, CD9 is composed of four transmembrane domains, short N- and C-terminal cytoplasmic domains, and two extracellular loops. The larger extracellular loop, referred to as the LEL or EC2, contains highly conserved CCG and PXSC motifs (2, 3). The LEL mediates noncovalent protein-protein interactions, allowing tetraspanins to associate with each other as well as signaling molecules, structural proteins, and G-protein coupled receptors (4-6). Human CD9 is expressed in multiple cell and tissue types and has been identified in diverse biological roles due to its involvement in the formation of tetraspanin-enriched microdomains (TEMs). TEMs are associated with numerous processes ranging from cell adhesion and fusion, membrane trafficking, and endocytosis to leukocyte adherence and motility (4-7). These tetraspanin-enriched microdomains (TEMs) are associated with a wide range of functions from cell adhesion and fusion, membrane trafficking and endocytosis, and eukocyte adherence and motility. The LEL of human CD9 shares 77% and 84% amino acid sequence identity with mouse and rat CD9, respectively.  CD9 can form homodimers or interact with other proteins including CD117, CD29, CD46, CD49c, CD81, CD315, Tspan4, TGF-alpha , and HBEGF (1, 4, 8-13). Increased expression of CD9 has been shown to enhance transmembrane TGF-alpha -induced EGFR stimulation (1), and injection of human CD9 mRNA into CD9 knock-out mouse oocytes restored sperm-egg fusion (14). CD9-LEL may also be involved in the inhibition of multinucleated giant cell formation (3) as well as possess anti-adhesive effects against bacteria trying to invade mammalian cells (6, 15). CD9 interacts with integrins to regulate cell adhesion and motility (16-18). CD9 has been implicated in platelet activation and aggregation (17, 19). It may act as the terminal signal of myelination in the peripheral nervous system and can regulate the formation of paranodal junctions (20). Also, it has been suggested CD9 plays an important role both in the self-antigen and recall antigen-induced T cell activation (21).
  1. Shi, W. et al. (2000) J. Cell Biol. 148:591.
  2. Hemler, M. (2003) Annu Rev Cell Biol. 19:397.
  3. Hulme, R. et al. (2014) PLoS One 9:e116289.
  4. Stipp, C. et al. (2003) Trends Biochem Sci. 28:106.
  5. Barreiro, O. et al. (2005) Blood 105:2852.
  6. Ventress, J. et al. (2016) PLoS One 11:e0160387.
  7. Rubinstein, E. (2011) Biochem Soc Trans. 39:501.
  8. Anzai, N. et al. (2002) Blood 99:4413.
  9. Radford, K. et al. (1996) Biochem. Biophys. Res. Commun. 222:13.
  10. Lozahic, S. et al. (2000) Eur. J. Immunol. 30:900.
  11. Park, K. et al. (2000) Mol. Hum. Reprod. 6:252.
  12. Charrin, S. et al. (2001) J. Biol. Chem. 276:14329.
  13. Tachibana, I. et al. (1997) J. Biol. Chem. 272:29181.
  14. Zhu, G. et al. (2002) Development 129:1995.
  15. Green, L. et al. (2011) Infect Immun. 79:2241.
  16. Powner, D. et al. (2011) Biochem. Soc. Trans. 39:563.
  17. Detchokul, S. et al. (2014) British Journal of Pharmacology 171:5462.
  18. Reyes, R. et al. (2018) Front. Immunol. 9:863.
  19. Slupsky, J. et al. (1989) J Biol chem. 264:12289.
  20. Ishibashi, T. et al. (2004) J. Neuroscience 24:96.
  21. Kobayashi, H. et al. (2004) Clin Exp Immunol. 137:101.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD9 Antibody (MAB25292)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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