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CD81 Antibody

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Western Blot: CD81 Antibody [NBP2-20564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at ...read more
Immunohistochemistry-Paraffin: CD81 Antibody [NBP2-20564] - CD81 Antibody Immunohistochemical analysis of paraffin-embedded Lung Papillary Adenocarcinoma, using antibody at 1:250 dilution.
Flow Cytometry: CD81 Antibody [NBP2-20564] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD81 antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated ...read more
Western Blot: CD81 Antibody [NBP2-20564] - AsPC-1 whole cell extracts (5.5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:500. The HRP-conjugated anti-rabbit ...read more
Western Blot: CD81 Antibody [NBP2-20564] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Pm, RM, ShSpecies Glossary
Applications WB, Flow, IHC, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD81 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.
Localization
Membrane
Predicted Species
Porcine (92%), Chimpanzee (100%), Rhesus Macaque (96%), Sheep (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD81
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-20564 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse (83%), Rat (86%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for CD81 Antibody

  • CD81 antigen (target of antiproliferative 1)
  • CD81 antigen
  • CD81 molecule
  • CD81
  • CVID6
  • TAPA1
  • TAPA-1
  • Target of the antiproliferative 1
  • Tetraspanin-28
  • TSPAN28
  • Tspan-28
  • tspan-28,26 kDa cell surface protein TAPA-1
  • TSPAN28S5.7

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD81 Antibody (NBP2-20564)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CD81 Antibody (NBP2-20564)

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Blogs on CD81.

Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma
By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is ...  Read full blog post.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

CD81/TAPA1: I'm on Tapa the Cell
Target of the antiproliferative 1 (TAPA1), also known as CD81, is found in the plasma membrane in lymphocytes and plays an important role in the regulation of lymphoma cell growth. This transmembrane 4 superfamily (TM4SF) protein is primarily found on...  Read full blog post.

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Bioinformatics

Gene Symbol CD81
Uniprot