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CD81 Antibody (M38) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Fe, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO, Single-Cell Western
Clone
M38
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-44861AF350
Alexa Fluor 405
NBP1-44861AF405
Alexa Fluor 488
NBP1-44861AF488
Alexa Fluor 532
NBP1-44861AF532
Alexa Fluor 594
NBP1-44861AF594
Alexa Fluor 647
NBP1-44861AF647
Alexa Fluor 700
NBP1-44861AF700
Alexa Fluor 750
NBP1-44861AF750
Allophycocyanin
NBP1-44861APC
Allophycocyanin/Cy7
NBP1-44861APCCY7
Biotin
NBP1-44861B
CoraFluor 1
NBP1-44861CL1
DyLight 350
NBP1-44861UV
DyLight 405
NBP1-44861V
DyLight 488
NBP1-44861G
DyLight 550
NBP1-44861R
DyLight 594
NBP1-44861DL594
DyLight 680
NBP1-44861FR
DyLight 755
NBP1-44861IR
FITC
NBP1-44859
HRP
NBP1-44861H
Janelia Fluor 525
NBP1-44861JF525
Janelia Fluor 549
NBP1-44861JF549
Janelia Fluor 585
NBP1-44861JF585
Janelia Fluor 635
NBP1-44861JF635
Janelia Fluor 646
NBP1-44861JF646
Janelia Fluor 669
NBP1-44861JF669
PE
NBP1-44861PE
PE/Atto594
NBP1-44861PEATT594
PE/Cy5.5
NBP1-44861PECY55
PE/Cy7
NBP1-44861PECY7
PerCP
NBP1-44861PCP
Unconjugated
NBP1-44861
mFluor Violet 450 SE
NBP1-44861MFV450
mFluor Violet 500 SE
NBP1-44861MFV500
mFluor Violet 610 SE
NBP1-44861MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-44861
BSA Free
NBP1-44861
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CD81 Antibody (M38) [DyLight 650] Summary

Immunogen
MOLT-4, human T-ALL cell line (NM_004356.3).
Specificity
The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD81
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Single Cell Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Cat (100%). Use in Mouse reported in scientific publication( PMID: 32573489).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD81 Antibody (M38) [DyLight 650]

  • CD81 antigen (target of antiproliferative 1)
  • CD81 antigen
  • CD81 molecule
  • CD81
  • CVID6
  • TAPA1
  • TAPA-1
  • Target of the antiproliferative 1
  • Tetraspanin-28
  • TSPAN28
  • Tspan-28
  • tspan-28,26 kDa cell surface protein TAPA-1
  • TSPAN28S5.7

Background

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD81 Antibody
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NBP1-44861C
CD81 Antibody (M38) [DyLight 650]
Species: Hu, Mu, Fe, Rb
Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO, Single-Cell Western

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Video Protocols

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Bioinformatics

Gene Symbol CD81