![Western Blot: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Western-Blot-NBP2-80660-img0002.jpg "Western Blot: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Gt, Pm, ShSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | 1D6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Clone 1D6 was used by HLDA to establish CD designation. |
| Immunogen | Aggregated OCI-Ly8 human B cell line |
| Localization | Membrane; Multi-pass membrane protein. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD81 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | NB100-65805 is a potent TAPA1/CD81 reagent, it induces homotypic adhesion and has powerful anti-proliferative effects. For Flow Cytometry: Use 10 uL of the suggested working dilution to label 106 cells in 100 uL. This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD81 Antibody (NBP2-80660)Find related products by research area.
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Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is ... Read full blog post. |
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Tools for Isolation, Quantification and Analysis of Exosomes Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat... Read full blog post. |
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CD19: An Undoubted Biomarker for B Cells CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t... Read full blog post. |
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CD81/TAPA1: I'm on Tapa the Cell Target of the antiproliferative 1 (TAPA1), also known as CD81, is found in the plasma membrane in lymphocytes and plays an important role in the regulation of lymphoma cell growth. This transmembrane 4 superfamily (TM4SF) protein is primarily found on... Read full blog post. |
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| Gene Symbol | CD81 |
![Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - The TAPA1 Antibody (1D6) was tested in A431 cells at a 1:10 dilution against DyLight 488 (green). Actin was counterstained against Phalloidin 568 (red) and cells were mounted in DAPI Flouromount (blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80660-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - The TAPA1 Antibody (1D6) was tested in A431 cells at a 1:10 dilution against DyLight 488 (green). Actin was counterstained against Phalloidin 568 (red) and cells were mounted in DAPI Flouromount (blue). Image from the standard format of this antibody.")
![Immunohistochemistry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80660-img0004.jpg "Immunohistochemistry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain. Image from the standard format of this antibody.")
![Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Staining of human peripheral blood lymphocytes. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80660-img0009.jpg "Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Staining of human peripheral blood lymphocytes. Image from the standard format of this antibody.")
![Western Blot: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Western-Blot-NBP2-80660-img0001.jpg "Western Blot: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL. Image from the standard format of this antibody.")
![Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80660-img0005.jpg "Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.")
![Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were directly conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80660-img0006.jpg "Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were directly conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).")
![Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80660-img0007.jpg "Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80660-img0008.jpg "Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.")
![Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80660-img0010.jpg "Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used. Image from the standard format of this antibody.")
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