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CD8 Antibody (569126) [Unconjugated]

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CD8a was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CD8a alpha Monoclonal Antibody (Catalog # MAB6709) at 25 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity CaSpecies Glossary
Applications ICC/IF
Clone
569126
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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CD8 Antibody (569126) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant canine CD8 alpha
Ser22-Glu186
Accession # P33706
Specificity
Detects canine CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant CD8 from human, mouse, cotton rat, cat, or guinea pig is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD8A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB6709 in the following application:

Publications
Read Publication using
MAB6709 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD8 Antibody (569126) [Unconjugated]

  • CD_antigen: CD8a
  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a molecule
  • CD8A
  • Leu2 T-lymphocyte antigen
  • LEU2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

Background

CD8 is a dimeric complex made up of two Ig superfamily members. The CD8 alpha chain is a 34 kDa type I transmembrane glycoprotein that is disulfide-linked either to itself forming CD8 alpha alpha , or to an unrelated 30‑35 kDa CD8 beta chain, forming CD8 alpha beta . CD8 alpha contains one V-type Ig-like domain in its extracellular region (ECD) that binds to class I MHC molecules. CD8 alpha beta is a TCR coreceptor, while CD8 alpha alpha promotes T cell survival and differentation. Within the ECD, canine CD8 alpha shares 63%, 45%, 43%, 68%, and 54% amino acid identity with human, mouse, rat, feline, and porcine CD8 alpha , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD8 Antibody (MAB6709)(1)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Filter By Species
Canine
(1)
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Review for CD8 Antibody (MAB6709) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using MAB6709:
Filter by Applications
ICC
(1)
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Filter by Species
Canine
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry CD8 MAB6709
Enlarge
5
reviewed by:
A G
ICC Canine 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedBlood lymphocytes
SpeciesCanine
LotCEQM0110121

Comments

CommentsAfter primary ab incubation, a secondary antibody attached to FITC fluorochrome was added to see fluroscence.

FAQs for CD8 Antibody (MAB6709) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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A G
05/26/2017
Application: ICC
Species: Canine

Bioinformatics

Gene Symbol CD8A
Uniprot