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CD69 Antibody [Unconjugated]

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CD69 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2359) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD69 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2359) for 3 hours at room ...read more
CD69 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Goat Anti-Human CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2359) at 3 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2359
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2359-SP
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CD69 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108
Specificity
Detects human CD69 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse CD69 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD69
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2359 in the following applications:
Publications
Read Publication using
AF2359 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD69 Antibody [Unconjugated]

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD69 Antibody (AF2359)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(1)
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Human
(1)
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Review for CD69 Antibody (AF2359) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2359:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD69 AF2359
Enlarge 		4
reviewed by:
Verified Customer
 IHC-P Human 03/03/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 heat-induced antigen retrieval

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Secondary Antibodies

 

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Immunohistochemistry-Paraffin CD69 AF2359
Enlarge
 Verified Customer
03/03/2019
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol CD69
Uniprot