CD63 Antibody (MEM-259) - BSA Free

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC, WB, Western Blot.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using NB100-77913	Applications	Species
Das S, Parray H, Chiranjivi A et al. Kennedy epitope (KE)-dependent retrograde transport of efficiently cleaved HIV-1 envelopes (Envs) and its effect on Env cell surface expression and viral particle formation Research Square 2022-11-10 (WB, Human)	WB	Human
Xianhui Ruan, Wei Yan, Minghui Cao, Ray Anthony M. Daza, Miranda Y. Fong, Kaifu Yang, Jun Wu, Xuxiang Liu, Melanie Palomares, Xiwei Wu, Arthur Li, Yuan Chen, Rahul Jandial, Nicholas C. Spitzer, Robert F. Hevner, Shizhen Emily Wang Breast cancer cell-secreted miR-199b-5p hijacks neurometabolic coupling to promote brain metastasis Nature Communications 2024-05-29 [PMID: 38811525]
Lee JY, Cho J, D'Egidio F et al. Probing Multiple Transplant Delivery Routes of CD+34 Stem Cells for Promoting Behavioral and Histological Benefits in Experimental Ischemic Stroke Stem cells translational medicine 2023-11-28 [PMID: 38016184] (IHC, Rat)	IHC	Rat
Supratik Das, Hilal Ahmad Parray, Adarsh Kumar Chiranjivi, Prince Kumar, Abhishek Goswami, Manish Bansal, Deepak Kumar Rathore, Rajesh Kumar, Sweety Samal Kennedy Epitope (KE)-dependent Retrograde Transport of Efficiently Cleaved HIV-1 Envelopes (Envs) and its Effect on Env Cell Surface Expression and Viral Particle Formation. The protein journal 2023-10-04 [PMID: 37794304]
Ruan X, Cao M, Yan W et al. Cancer-cell-secreted extracellular vesicles target p53 to impair mitochondrial function in muscle EMBO reports 2023-07-13 [PMID: 37439436]
Carpenter MA, Ginugu M, Khan S, Kemp MG DNA containing cyclobutane pyrimidine dimers is released from UVB-irradiated keratinocytes in a caspase-dependent manner The Journal of investigative dermatology 2022-06-09 [PMID: 35691362]
Zhang Z, Hu J, Ishihara M et al. The miRNA-21-5p Payload in Exosomes from M2 Macrophages Drives Tumor Cell Aggression via PTEN/Akt Signaling in Renal Cell Carcinoma International journal of molecular sciences 2022-03-10 [PMID: 35328425] (WB, Human)	WB	Human
Gustafson K, Huynh K, Heineck D et al. Automated fluorescence quantification of extracellular vesicles collected from blood plasma using dielectrophoresis Lab on a Chip 2021-03-24 [PMID: 33877235] (ICC/IF, Human)	ICC/IF	Human
Henrich SE, McMahon KM, Plebanek MP et al. Prostate cancer extracellular vesicles mediate intercellular communication with bone marrow cells and promote metastasis in a cholesterol-dependent manner Journal of Extracellular Vesicles 2020-12-31 [PMID: 33408816] (Western Blot)	Western Blot
Dinh PC, Paudel D, Brochu H et al. Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis Nat Commun 4389-01-01 [PMID: 32111836] (WB, Human)	WB	Human
Cumba Garcia L, Peterson T, Cepeda M et al. Isolation and Analysis of Plasma-Derived Exosomes in Patients With Glioma Front. Oncol. 2019-07-16 [PMID: 31380286] (WB, Human)	WB	Human
Fong MY, Zhou W, Liu L et al. Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis Nat. Cell Biol. 2015-02-01 [PMID: 25621950] (IF/IHC, Human) Details: Wang SE	IF/IHC	Human
Vickers K C, Palmisano B T et al. MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol 2011-01-04 [PMID: 21423178] (WB, Human)	WB	Human
Kishimoto T et al. Leucocyte Typing VI. Garland Publishing Inc. London.
Chow A, Zhou W, Liu L et al. Macrophage immunomodulation by breast cancer-derived exosomes requires Toll-like receptor 2-mediated activation of NF-kB. Sci Rep 2014-07-18 [PMID: 25034888] (WB, Human) Details: CD63 antibody used for WB on exosomes derived from MCF10A, MDA-MB-231 and MCF7 cells (Figure 1b; Supplementary Fig. S3).	WB	Human
Yoon SO et al. Am. J. Pathol. 177:377. 2010-01-01 [PMID: 20566742]
Schmidt H et al. BMC Immunol. 10:41. 2009 [PMID: 19640298] (WB)	WB
Wang X et al. Infect. Immun. 74:1873. 2006 [PMID: 16495562] (FLOW)	FLOW
Show All 18 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IF	Human	10/23/2019	View Summary Application Immunofluorescence Sample Tested hela cell Species Human Lot 529390

Array NB100-77913

• CD63 Antibodies
• CD63 ELISA Kit
• CD63 Lysate
• CD63 Proteins
• CD63 Proteins and Enzyme