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CD59 Antibody (MEM-43/5) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clone
MEM-43/5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-400AF350
Alexa Fluor 405
NB500-400AF405
Alexa Fluor 488
NB500-400AF488
Alexa Fluor 532
NB500-400AF532
Alexa Fluor 594
NB500-400AF594
Alexa Fluor 647
NB500-400AF647
Alexa Fluor 700
NB500-400AF700
Alexa Fluor 750
NB500-400AF750
Allophycocyanin
NB500-400APC
Allophycocyanin/Cy7
NB500-400APCCY7
Biotin
NB500-400B
CoraFluor 1
NB500-400CL1
DyLight 350
NB500-400UV
DyLight 405
NB500-400V
DyLight 488
NB500-400G
DyLight 550
NB500-400R
DyLight 594
NB500-400DL594
DyLight 650
NB500-400C
DyLight 680
NB500-400FR
DyLight 755
NB500-400IR
FITC
NB500-400F
HRP
NB500-400H
Janelia Fluor 549
NB500-400JF549
Janelia Fluor 585
NB500-400JF585
Janelia Fluor 635
NB500-400JF635
Janelia Fluor 646
NB500-400JF646
Janelia Fluor 669
NB500-400JF669
PE
NB500-400PE
PE/Atto594
NB500-400PEATT594
PE/Cy5.5
NB500-400PECY55
PE/Cy7
NB500-400PECY7
PerCP
NB500-400PCP
Unconjugated
NB500-400
mFluor Violet 450 SE
NB500-400MFV450
mFluor Violet 500 SE
NB500-400MFV500
mFluor Violet 610 SE
NB500-400MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-400
BSA Free
NB500-400
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CD59 Antibody (MEM-43/5) [Janelia Fluor® 525] Summary

Additional Information
Clone MEM-43/5 was used by HLDA to establish CD designation.
Immunogen
Thymocytes and T lymphocytes.
Specificity
The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V; WS Code AS S012
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD59
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD59 Antibody (MEM-43/5) [Janelia Fluor® 525]

  • 16.3A5
  • 1F5 antigen
  • 1F5
  • 20 kDa homologous restriction factor
  • CD59 antigen
  • CD59 antigen, complement regulatory protein
  • CD59 glycoprotein
  • CD59 molecule, complement regulatory protein
  • CD59
  • EJ16
  • EJ30
  • EJ30, EL32 and G344)
  • EL32
  • FLJ38134
  • FLJ92039
  • G344
  • HRF20
  • HRF-20
  • human leukocyte antigen MIC11
  • Ly-6-like protein
  • lymphocytic antigen CD59/MEM43
  • MACIF
  • MAC-inhibitory protein
  • MAC-IP
  • MEM43 antigen
  • MEM43
  • membrane attack complex (MAC) inhibition factor
  • Membrane attack complex inhibition factor
  • Membrane inhibitor of reactive lysis
  • MGC2354
  • MIC11
  • MIC11MSK21
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • p18-20
  • Protectin
  • surface anitgen recognized by monoclonal 16.3A5
  • T cell-activating protein

Background

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD59