![Flow Cytometry: CD59 Antibody (MEM-43/5) [Allophycocyanin] [NB500-400APC] - The PIGAc.1234C>T mutation increases PIGA function compared to PIGAnull hiPSCs.(A). Representative example of FACS analysis CD59 expression in the three hiPSC lines. Overlay histogram shows that CD59 expression was significantly higher in PIGAc.1234 C>T hiPSCs compared to PIGAnull hiPSCs. MFI was 445.4 in PIGAwt hiPSCs and 332.6 in PIGAc.1234C>T hiPSCs (p>0.05, NS). However, MFI in PIGAc.1234C>T hiPSCs was significantly higher than 17.9 in PIGAnull hiPSCs (*pT hiPSCs. PIGAnull hiPSCs (purple), PIGAc.1234C>T hiPSCs (orange) and PIGAwt hiPSCs (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Allophycocyanin-Flow-Cytometry-NB500-400APC-img0002.jpg "Flow Cytometry: CD59 Antibody (MEM-43/5) [Allophycocyanin] [NB500-400APC] - The PIGAc.1234C>T mutation increases PIGA function compared to PIGAnull hiPSCs.(A). Representative example of FACS analysis CD59 expression in the three hiPSC lines. Overlay histogram shows that CD59 expression was significantly higher in PIGAc.1234 C>T hiPSCs compared to PIGAnull hiPSCs. MFI was 445.4 in PIGAwt hiPSCs and 332.6 in PIGAc.1234C>T hiPSCs (p>0.05, NS). However, MFI in PIGAc.1234C>T hiPSCs was significantly higher than 17.9 in PIGAnull hiPSCs (*pT hiPSCs. PIGAnull hiPSCs (purple), PIGAc.1234C>T hiPSCs (orange) and PIGAwt hiPSCs (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, IP, CyTOF-ready |
| Clone | MEM-43/5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Allophycocyanin |
| Additional Information | Clone MEM-43/5 was used by HLDA to establish CD designation. |
| Immunogen | Thymocytes and T lymphocytes. |
| Specificity | The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V; WS Code AS S012 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD59 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
![CD59 Antibody (MEM-43/5) [Allophycocyanin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "CD59 Antibody (MEM-43/5) [Allophycocyanin]")
Secondary Antibodies |
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![Flow Cytometry: CD59 Antibody (MEM-43/5) [Allophycocyanin] [NB500-400APC] - Generation of PIGAc.1234C>T mutation using the PiggyBac transposon system. Representative FACS analysis CD59 expression in TF1PIGAnull cells transfected with PB-PIGAwt, PB-PIGAc.1234C>T or PB-PIGAtr411. Transfected TF1PIGAnull cells were stained with an APC-conjugated CD59 antibody to assess PIGA gene expression. Non-transfected TF1PIGAnull cells were used as a control. MFI represents mean fluorescence intensity. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Allophycocyanin-Flow-Cytometry-NB500-400APC-img0001.jpg "Flow Cytometry: CD59 Antibody (MEM-43/5) [Allophycocyanin] [NB500-400APC] - Generation of PIGAc.1234C>T mutation using the PiggyBac transposon system. Representative FACS analysis CD59 expression in TF1PIGAnull cells transfected with PB-PIGAwt, PB-PIGAc.1234C>T or PB-PIGAtr411. Transfected TF1PIGAnull cells were stained with an APC-conjugated CD59 antibody to assess PIGA gene expression. Non-transfected TF1PIGAnull cells were used as a control. MFI represents mean fluorescence intensity. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license.")
![Flow Cytometry: CD59 Antibody (MEM-43/5) [Allophycocyanin] [NB500-400APC] - The PIGAc.1234C>T mutation does not impair terminal hematopoietic differentiation during mesoderm induction. Representative example of FACS analysis of hematopoietic phenotypes in the EB-BLCs from PIGAwt and PIGAc.1234C>T. The zebra plot shows expression of CD59 (X-axis) and CD45 (Y-axis) after three and eight days of hematopoietic differentiation. Unstained PIGAwt cells were used as a control. Abbreviations: CFU-Macrophage (M); CFU-Granulocyte-Macrophage (GM); committed erythroid BFU-E (BFU) and CFU-E (CFU) progenitors; multipotent progenitor cells CFU-GEMM (GEMM). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Allophycocyanin-Flow-Cytometry-NB500-400APC-img0003.jpg "Flow Cytometry: CD59 Antibody (MEM-43/5) [Allophycocyanin] [NB500-400APC] - The PIGAc.1234C>T mutation does not impair terminal hematopoietic differentiation during mesoderm induction. Representative example of FACS analysis of hematopoietic phenotypes in the EB-BLCs from PIGAwt and PIGAc.1234C>T. The zebra plot shows expression of CD59 (X-axis) and CD45 (Y-axis) after three and eight days of hematopoietic differentiation. Unstained PIGAwt cells were used as a control. Abbreviations: CFU-Macrophage (M); CFU-Granulocyte-Macrophage (GM); committed erythroid BFU-E (BFU) and CFU-E (CFU) progenitors; multipotent progenitor cells CFU-GEMM (GEMM). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license.")
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