Reactivity | HuSpecies Glossary |
Applications | Flow, ICC/IF, CyTOF-ready, ICC/IF |
Clone | 143-30 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | CoraFluor 1 |
Description | CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays. |
Additional Information | Clone 143-30 was used by HLDA to establish CD designation. |
Immunogen | PHA stimulated human CD55/DAF (Uniprot: P08174 ) |
Localization | Cell Surface |
Specificity | Recognizes a single chain glycoprotein of 70kDa, identified as CD55/DAF. It is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The monoclonal antibody 143-30 may be used as marker for paroxysmal nocturnal hemoglobinuria (PNH). |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | CD55 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. Do not freeze. |
Buffer | PBS |
Preservative | No Preservative |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD55/DAF Antibody (NBP2-47964CL1)Find related products by research area.
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Gene Symbol | CD55 |