Home » CD55/DAF » CD55/DAF Antibodies » CD55/DAF Antibody (143-30) - Azide and BSA Free

CD55/DAF Antibody (143-30) - Azide and BSA Free

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Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (143-30) - Azide and BSA Free [NBP2-47964] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.
Immunocytochemistry/ Immunofluorescence: CD55/DAF Antibody (143-30) - Azide and BSA Free [NBP2-47964] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat ...read more
Flow Cytometry: CD55/DAF Antibody (143-30) - Azide and BSA Free [NBP2-47964] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 (Blue) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, CyTOF-ready, ICC/IF
Clone
143-30
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47964AF350
Alexa Fluor 405
NBP2-47964AF405
Alexa Fluor 488
NBP2-47964AF488
Alexa Fluor 532
NBP2-47964AF532
Alexa Fluor 594
NBP2-47964AF594
Alexa Fluor 647
NBP2-47964AF647
Alexa Fluor 700
NBP2-47964AF700
Alexa Fluor 750
NBP2-47964AF750
Allophycocyanin
NBP2-47964APC
Allophycocyanin/Cy7
NBP2-47964APCCY7
Biotin
NBP2-47964B
CoraFluor 1
NBP2-47964CL1
DyLight 350
NBP2-47964UV
DyLight 405
NBP2-47964V
DyLight 488
NBP2-47964G
DyLight 550
NBP2-47964R
DyLight 594
NBP2-47964DL594
DyLight 650
NBP2-47964C
DyLight 680
NBP2-47964FR
DyLight 755
NBP2-47964IR
FITC
NBP2-47964F
Janelia Fluor 525
NBP2-47964JF525
Janelia Fluor 549
NBP2-47964JF549
Janelia Fluor 646
NBP2-47964JF646
Janelia Fluor 669
NBP2-47964JF669
PE
NBP2-47964PE
PE/Atto594
NBP2-47964PEATT594
PE/Cy5.5
NBP2-47964PECY55
PE/Cy7
NBP2-47964PECY7
PerCP
NBP2-47964PCP
Unconjugated
NBP2-44873-0.1mg
mFluor Violet 450 SE
NBP2-47964MFV450
mFluor Violet 500 SE
NBP2-47964MFV500
mFluor Violet 610 SE
NBP2-47964MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44873-0.1mg
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CD55/DAF Antibody (143-30) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44873).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Additional Information
Clone 143-30 was used by HLDA to establish CD designation.
Immunogen
PHA stimulated human CD55/DAF (Uniprot: P08174 )
Localization
Cell Surface
Specificity
Recognizes a single chain glycoprotein of 70kDa, identified as CD55/DAF. It is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The monoclonal antibody 143-30 may be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD55
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CD55/DAF Antibody (143-30) - Azide and BSA Free

  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • CD55
  • CR
  • CRdecay accelerating factor for complement (CD55, Cromer blood group system)
  • CROMDAFcomplement decay-accelerating factor
  • DAF
  • decay accelerating factor for complement
  • TC

Background

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD55