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CD52 Antibody (HI186) - BSA Free

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Flow Cytometry: CD52 Antibody (HI186) [NB500-311] - Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE). Fig. 1A CD52 positive cALL (gate leukemic cells).
Flow Cytometry: CD52 Antibody (HI186) [NB500-311] - Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE). Fig. 1A CD52 ...read more
Flow Cytometry: CD52 Antibody (HI186) [NB500-311] - Fig.1B CD52 negative pro T ALL (gate leukemic cells)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
HI186
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-311AF350
Alexa Fluor 405
NB500-311AF405
Alexa Fluor 488
NB500-311AF488
Alexa Fluor 532
NB500-311AF532
Alexa Fluor 594
NB500-311AF594
Alexa Fluor 647
NB500-311AF647
Alexa Fluor 700
NB500-311AF700
Alexa Fluor 750
NB500-311AF750
Allophycocyanin
NB500-311APC
Allophycocyanin/Cy7
NB500-311APCCY7
Biotin
NB500-311B
CoraFluor 1
NB500-311CL1
DyLight 350
NB500-311UV
DyLight 405
NB500-311V
DyLight 488
NB500-311G
DyLight 550
NB500-311R
DyLight 594
NB500-311DL594
DyLight 650
NB500-311C
DyLight 680
NB500-311FR
DyLight 755
NB500-311IR
FITC
NB500-311F
HRP
NB500-311H
Janelia Fluor 525
NB500-311JF525
Janelia Fluor 549
NB500-311JF549
Janelia Fluor 585
NB500-311JF585
Janelia Fluor 635
NB500-311JF635
Janelia Fluor 646
NB500-311JF646
Janelia Fluor 669
NB500-311JF669
PE
NB500-311PE
PE/Atto594
NB500-311PEATT594
PE/Cy5.5
NB500-311PECY55
PE/Cy7
NB500-311PECY7
PerCP
NB500-311PCP
mFluor Violet 450 SE
NB500-311MFV450
mFluor Violet 500 SE
NB500-311MFV500
mFluor Violet 610 SE
NB500-311MFV610
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CD52 Antibody (HI186) - BSA Free Summary

Additional Information
Clone HI186 was used by HLDA to establish CD designation.
Immunogen
Human tonsil (NM_001803.2).
Specificity
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract. HLDA VI; WS Code BP 523 HLDA VI; WS Code T 6T-057
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD52
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD52 Antibody (HI186) - BSA Free

  • Cambridge pathology 1 antigen
  • CAMPATH-1 antigen
  • CAMPATH-1
  • CD52 antigen (CAMPATH-1 antigen)
  • CD52 antigen
  • CD52 molecule
  • CD52
  • CDW52 antigen (CAMPATH-1 antigen)
  • CDw52
  • Epididymal secretory protein E5
  • He5
  • HEL-S-171mP
  • Human epididymis-specific protein 5

Background

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD52
Entrez
Uniprot