CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free

Images

 
Western Blot: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924] - Western Blot Analysis of human spleen cell lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).
Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD45RB Rabbit Recombinant Monoclonal Antibody ...read more
Flow Cytometry: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R) followed ...read more
Western Blot: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924] - Western Blot Analysis of human Raji cell lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).

Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
PTPRC/2877R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07180).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant human full-length CD45RB protein (Uniprot: P08575)
Localization
Cell Surface
Marker
B-Cell Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Reactivity Notes

Does not react with Rat or Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free

  • B220
  • CD45 antigen
  • CD45R
  • CD45RB
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45RB is an alternatively spliced form of CD45, a cell surface glycoprotein. It is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PTPRC