CD45 Antibody (30-F11) - BSA Free

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, is a member C of the class 1 receptor-like protein tyrosine phosphatase (PTPRC) family (1, 2). It is a transmembrane glycoprotein which, due to alternative splicing, has a multiple isoforms with a theoretical molecular weight ranging from 180 - 220 kDa (1, 3-5). Human CD45 is synthesized as a 1281 amino acid sequence consisting of an alternatively spliced extracellular receptor-like region, a cysteine-rich domain, fibronectin-like III repeats, a transmembrane segment, and a cytoplasmic region with tandem protein tyrosine phosphatase (PTP) domains: the membrane proximal domain (D1) and the membrane distal domain (D2) (3, 5). CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5). More specifically, CD45 positively regulates antigen receptor signaling and Src-family member kinase activity (1, 3). There are many ways to regulate CD45 phosphatase activity including ligand binding, dimerization, protein interactions, cellular localization, and covalent modifications (3, 6). Ligands for CD45 include pUL11, a transmembrane protein of the cytomegalovirus RL11 (CMV RL11) family, and placental protein 14 (PP14), both of which exclusively bind CD45, and various lectins including CD22, galectin-1, galectin-3, macrophage mannose receptor (MR), and macrophage galactose-type lectin (MGL) (6).

Given its role in immune cell development and activation, CD45 has also been linked to a variety of diseases. The importance of CD45 in immunity has been revealed in human and mouse studies where CD45-deficiency leads to a severe-combined immunodeficiency (SCID) phenotype (2, 3, 6). A CD45-knockout mice study revealed inhibited thymocyte production and poor B-cell response, whereas CD45 activation in mice causes lymphoproliferation and autoantibody production (3). CD45 variants have been associated with altered immune function and autoimmune disorders including multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (6). Furthermore, altered CD45 expression has been implicated in oncological conditions including chronic lymphatic leukemia, acute lymphatic leukemia, Hodgkin lymphoma, multiple myeloma, and diffuse large B-cell lymphoma (6). Considering its role in autoimmune disorders, immunodeficiency and cancer, CD45 is an ideal therapeutic target (3, 6). The main approaches to control CD45 function is through either selective inhibitors or anti-CD45 antibodies (3).

Alternative names for CD45 includes B220, CD antigen: CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, LCA, Leukocyte common antigen, LY5, protein tyrosine phosphatase receptor type c polypeptide, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 Glycoprotein, and T200.

References

1. Trowbridge, I. S., & Thomas, M. L. (1994). CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annual review of immunology. https://doi.org/10.1146/annurev.iy.12.040194.000505

2. Andersen, J. N., Jansen, P. G., Echwald, S. M., Mortensen, O. H., Fukada, T., Del Vecchio, R., Tonks, N. K., & Moller, N. P. (2004). A genomic perspective on protein tyrosine phosphatases: gene structure, pseudogenes, and genetic disease linkage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

3. Hermiston, M. L., Xu, Z., & Weiss, A. (2003). CD45: a critical regulator of signaling thresholds in immune cells. Annual review of immunology. https://doi.org/10.1146/annurev.immunol.21.120601.140946

4. Tonks, N. K., Diltz, C. D., & Fischer, E. H. (1990). CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. The Journal of biological chemistry.

5. Nam, H. J., Poy, F., Saito, H., & Frederick, C. A. (2005). Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. The Journal of experimental medicine. https://doi.org/10.1084/jem.20041890

6. Rheinlander, A., Schraven, B., & Bommhardt, U. (2018). CD45 in human physiology and clinical medicine. Immunology letters. https://doi.org/10.1016/j.imlet.2018.01.009

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 12 applications: FLOW, Flow, Flow Cytometry, Func, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, In Vivo, In vitro, WB.

Showing Publications 1 - 10 of 45. Show All 45 Publications. Collapse Publications.

Publications using NB100-77417	Applications	Species
Andreas Frøslev Mathisen, Ulrik Larsen, Natalie Kavli, Lucas Unger, Laura Maria Daian, Andrei Mircea Vacaru, Ana-Maria Vacaru, Pedro Luis Herrera, Luiza Ghila, Simona Chera Moderate beta-cell ablation triggers synergic compensatory mechanisms even in the absence of overt metabolic disruption Communications Biology 2024-07-09 [PMID: 38982170]
Yang M, Ong J, Meng F et al. Spatiotemporal insight into early pregnancy governed by immune-featured stromal cells Cell 2023-09-07 [PMID: 37699390]
Klak M, Wszo?a M, Berman A et al. Bioprinted 3D Bionic Scaffolds with Pancreatic Islets as a New Therapy for Type 1 Diabetes-Analysis of the Results of Preclinical Studies on a Mouse Model Journal of functional biomaterials 2023-07-14 [PMID: 37504866] (IHC, Mouse)	IHC	Mouse
Reches G, Blondheim Shraga NR, Carrette F et al. Resolving the conflicts around Par2 opposing roles in regeneration by comparing immune-mediated and toxic-induced injuries Inflammation and regeneration 2022-11-29 [PMID: 36447218] (IHC-P, Mouse)	IHC-P	Mouse
Wu M, Yu Z, Matar DY et al. Human Amniotic Membrane Promotes Angiogenesis in an Oxidative Stress Chronic Diabetic Murine Wound Model Advances in wound care 2022-03-16 [PMID: 35293255] (IF/IHC, Mouse)	IF/IHC	Mouse
Yusuf AM, Qaisar R, Al-Tamimi AO Et al. Cardiomyocyte-GSK-3beta deficiency induces cardiac progenitor cell proliferation in the ischemic heart through paracrine mechanisms Journal of cellular physiology 2021-11-23 [PMID: 34812500]
George KK, Heithoff BP, Shandra O, Robel S Mild traumatic brain injury/ concussion initiates an atypical astrocyte response caused by blood-brain barrier dysfunction Journal of neurotrauma 2021-11-22 [PMID: 34806422]
Everton E, Rizvi F, Smith AR Et al. Transient yet Robust Expression of Proteins in the Mouse Liver via Intravenous Injection of Lipid Nanoparticle-encapsulated Nucleoside-modified mRNA Bio-protocol 2021-10-05 [PMID: 34722830] (IHC-Fr)	IHC-Fr
Wen S, Deng F, Li L et al. VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation Journal of diabetes investigation 2021-09-08 [PMID: 34494385] (IHC-P, WB, Human, Mouse)	IHC-P, WB	Human, Mouse
Wutschka J, Kast B, Sator-Schmitt M Et al. JUNB suppresses distant metastasis by influencing the initial metastatic stage Clin Exp Metastasis 2021-07-20 [PMID: 34282521] (WB, Mouse)	WB	Mouse
Wang J, Kural M, Wu J et al. An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies Biomaterials 2021-08-01 [PMID: 34087584]
Jelinkova S, Sleiman Y, FojtIk P et al. Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart International Journal of Molecular Sciences 2021-05-10 [PMID: 34068508] (IF/IHC, Mouse)	IF/IHC	Mouse
Gangoso E, Southgate B, Bradley L et al. Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion Cell 2021-04-29 [PMID: 33857425] (IF/IHC, Mouse)	IF/IHC	Mouse
Moiseenko A, Vazquez-Armendariz AI, Kheirollahi V et al. Identification of a Repair-Supportive Mesenchymal Cell Population during Airway Epithelial Regeneration Cell reports 2020-12-22 [PMID: 33357434] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (FLOW, Mouse) Details: Citation using the PE format of this antibody.	FLOW	Mouse
Sharifpanah F, Ali EH, Wartenberg M, Sauer H. The milk thistle (Silybum marianum) compound Silibinin stimulates leukopoiesis from mouse embryonic stem cells Phytother Res 2018-12-11 [PMID: 30548344] (WB, Mouse)	WB	Mouse
Pei G, Yang L, Li J Et al. Sensory nerves directly promote osteoclastogenesis by secreting Cyp40 Research Square 2021-11-23 (IF/IHC, Rat)	IF/IHC	Rat
Jakubowska MA, Pyka J, Michalczyk-Wetula D et al. Electron paramagnetic resonance spectroscopy reveals alterations in the redox state of endogenous copper and iron complexes in photodynamic stress-induced ischemic mouse liver Redox Biol 2020-05-12 [PMID: 32464500] (IF/IHC, Mouse)	IF/IHC	Mouse
Simanjuntak Y, Ko HY, Lee YL et al. Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice PLoS Pathog. 2020-05-01 [PMID: 32392268] (IF/IHC, Mouse)	IF/IHC	Mouse
Chen BY Image-guided therapeutic intervention in autoimmune diseases Thesis 2022-01-01 (IF/IHC, Mouse)	IF/IHC	Mouse
Li B, Luan S, Chen J et al. The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p Molecular Therapy - Nucleic Acids 2020-03-06 [PMID: 31958697] (Flow Cytometry)	Flow Cytometry
Chen BY, Ghezzi C, Villegas B et al. 18F-FAC PET visualizes brain-infiltrating leukocytes in a mouse model of multiple sclerosis J. Nucl. Med. 2019-10-25 [PMID: 31653711] (IF/IHC)	IF/IHC
Chang CS, Liao CC, Liou AT et al. Enterovirus 71 targets the cardiopulmonary system in a robust oral infection mouse model Sci Rep 2019-07-31 [PMID: 31366973] (IF/IHC, Mouse)	IF/IHC	Mouse
Braun RK, Chetty C, Balasubramaniam V, et al Intraperitoneal injection of MSC-derived exosomes prevent experimental bronchopulmonary dysplasia. Biochem Biophys Res Commun. 2018 Aug 07 [PMID: 30093115] (Flow, Rat)	Flow	Rat
Kramann R, Machado F, Wu H et al. Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis. JCI Insight 2018-05-03 [PMID: 29720573]
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018-01-24 [PMID: 29367423] (FLOW, Mouse) Details: This citation used the Alexa Fluor 488 form of this antbody	FLOW	Mouse
Caballero S, Kent DL, Sengupta N et al. Bone Marrow-Derived Cell Recruitment to the Neurosensory Retina and Retinal Pigment Epithelial Cell Layer Following Subthreshold Retinal Phototherapy. Invest. Ophthalmol. Vis. Sci. 2017-10-01 [PMID: 29049716] (IF/IHC, Mouse)	IF/IHC	Mouse
Khoriaty R, Vogel N, Hoenerhoff MJ et al. SEC23B is required for pancreatic acinar cell function in adult mice. Mol. Biol. Cell 2017-05-24 [PMID: 28539403] (IF/IHC, Mouse)	IF/IHC	Mouse
Ambrosi TH, Scialdone A, Graja A et al. Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration Cell Stem Cell 2017-03-24 [PMID: 28330582] (FLOW, Mouse)	FLOW	Mouse
Mendes VI, Bartholomeusz GA, Ayres M et al. Synthesis and cytotoxic activity of novel A-ring cleaved ursolic acid derivatives in human non-small cell lung cancer cells Eur J Med Chem 2016-07-22 [PMID: 27484517] (IF/IHC, Mouse)	IF/IHC	Mouse
Celik Mo, Labuz D, Henning K et al. Leukocyte opioid receptors mediate analgesia via Ca(2+)-regulated release of opioid peptides. Brain Behav. Immun. 2016-04-30 [PMID: 27139929] (ICC/IF, Mouse)	ICC/IF	Mouse
De Ponti A, Wiechert L, Stojanovic A et al. Chronic liver inflammation and hepatocellular carcinogenesis are independent of S100A9. Int. J. Cancer. 2014-10-21 [PMID: 25331529] (IHC-P, Mouse)	IHC-P	Mouse
Tsuboi S, Fukuda M. Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs humoral immune responses in transgenic mice.. J Biol Chem. 1998-11-13 [PMID: 9804842] (WB)	WB
Keller G, Wall C, Fong AZ, Hawley TS et al. Overexpression of HOX11 leads to the immortalization of embryonic precursors with both primitive and definitive hematopoietic potential.. Blood. 1998-08-01 [PMID: 9680355] (FLOW)	FLOW
Maroun CR, Julius M. Distinct involvement of CD45 in antigen receptor signalling in CD4+ and CD8+ primary T cells.. Eur J Immunol. 1994-04-01 [PMID: 7908636]
Hanaoka K, Fujita N, Lee SH et al. Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cells.. Cancer Res. 1995-05-15 [PMID: 7743522] (Func, Mouse) Details: CD45 antibody (clone 30-F11) was used for studying the effects of anti-CD45 antibody in vitro on murine CS-21 lymphoma cells. The parameters studied were adhesion (Fig. 6) and apoptosis (Fig. 7).	Func	Mouse
Pei G, Yang L, Liu B et al. Sensory nerves directly promote osteoclastogenesis by secreting Peptidyl-prolyl cis-trans isomerase D (Cyp40) Research Square 2023-05-08 (IHC-Fr, Mouse) Details: IHC-Fr 1:100	IHC-Fr	Mouse
Condiff EK The Role of Laminin in a Model of Maternal Antiviral Immune Activation During Early Placental Development Thesis 2023-01-01
Seaman WE, Talal N, Herzenberg LA et al. Surface antigens on mouse natural killer cells: use of monoclonal antibodies to inhibit or to enrich cytotoxic activity.. J Immunol. 1981-09-01 [PMID: 6790624]
Ralph SJ, Berridge MV et al. Expression of antigens of the 'T200' family of glycoproteins on hemopoietic stem cells: evidence that thymocyte cell lineage antigens are represented on 'T200'.. J Immunol. 1984-05-01 [PMID: 6425407] (Func, Mouse)	Func	Mouse
Seaman WE, Wofsy D, Greenspan JS, Ledbetter JA. Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease.. J Immunol. 1983-04-01 [PMID: 6187819] (In Vivo, Mouse)	In Vivo	Mouse
Raveche ES, Chused TM, Steinberg AD et al. Comparison of response to stem cell differentiation signals between normal and autoimmune mouse strains.. J Immunol. 1985-02-01 [PMID: 3880791] (FLOW, Mouse)	FLOW	Mouse
Gutstein NL, Seaman WE, Scott JH et al. Induction of immune tolerance by administration of monoclonal antibody to L3T4.. J Immunol. 1986-08-15 [PMID: 3090139] (In Vivo, Mouse)	In Vivo	Mouse
Hayakawa K, Carmack CE, Hyman R, Hardy RR. Natural autoantibodies to thymocytes: origin, VH genes, fine specificities, and the role of Thy-1 glycoprotein.. J Exp Med. 1990-09-01 [PMID: 1974916] (In vitro, Mouse)	In vitro	Mouse
Matthews DC, Badger CC, Fisher DR et al. Selective radiation of hematolymphoid tissue delivered by anti-CD45 antibody.. Cancer Res. 1992-03-01 [PMID: 1531324] (In Vivo, Mouse)	In Vivo	Mouse
Show All 45 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-P	Mouse	10/13/2021	View Summary Application Immunohistochemistry-Paraffin Sample Tested glomeruli Species Mouse Lot 30-F11 Comments Comments 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. 1ry antibody dilution 1:100, 2ry antibody 1:400
Enlarge	5 reviewed by: Verified Customer	IHC-P	Mouse	09/15/2017	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse spleen cells Species Mouse Lot B-13 Comments Comments No permeabilization Antigen retrieval: Citrate buffer, 20 minutes, 90C 1st ab dilution: 1:100
	5 reviewed by: Verified Customer	IF	Rat	11/24/2015	View Summary Application Immunofluorescence Sample Tested Mouse Spleen Species Rat
	5 reviewed by: Verified Customer	IHC	Mouse	10/02/2014	View Summary Application Immunohistochemistry Sample Tested mouse liver Species Mouse
Enlarge	4 reviewed by: Aurora De Ponti	IF	Mouse	09/05/2014	View Summary Application Immunofluorescence Sample Tested mouse liver Species Mouse Lot B144981

Array NB100-77417

• CD45 Antibodies
• CD45 Lysate
• CD45 Proteins
• CD45 Proteins and Enzymes
• CD45 ELISA