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CD43/Sialophorin Antibody (W3/13) - BSA Free

 (3 Publications)
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Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD43 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.
Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 ...read more
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - CD43 was detected in immersion fixed paraffin-embedded sections of rat thymus using Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # ...read more
Flow Cytometry: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Staining of rat splenocytes using CD43 FITC.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, IHC
Clone
W3/13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-64992AF350
Alexa Fluor 405
NB100-64992AF405
Alexa Fluor 488
NB100-64992AF488
Alexa Fluor 532
NB100-64992AF532
Alexa Fluor 594
NB100-64992AF594
Alexa Fluor 647
NB100-64992AF647
Alexa Fluor 700
NB100-64992AF700
Alexa Fluor 750
NB100-64992AF750
Allophycocyanin
NB100-64992APC
Biotin
NB100-64992B
DyLight 350
NB100-64992UV
DyLight 405
NB100-64992V
DyLight 488
NB100-64992G
DyLight 550
NB100-64992R
DyLight 594
NB100-64992DL594
DyLight 650
NB100-64992C
DyLight 680
NB100-64992FR
DyLight 755
NB100-64992IR
FITC
NB100-64992F
HRP
NB100-64992H
Janelia Fluor 525
NB100-64992JF525
Janelia Fluor 549
NB100-64992JF549
Janelia Fluor 585
NB100-64992JF585
Janelia Fluor 635
NB100-64992JF635
Janelia Fluor 646
NB100-64992JF646
Janelia Fluor 669
NB100-64992JF669
PE
NB100-64992PE
PerCP
NB100-64992PCP
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CD43/Sialophorin Antibody (W3/13) - BSA Free Summary

Immunogen
Rat thymocyte membrane glycoproteins
Localization
Type I membrane protein.
Specificity
Rat CD43 cell surface antigen.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:25-1:100
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5 ug/ml
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use in Western blot reported in scientific literature (PMID 7504163).
Publications
Read Publications using
NB100-64992 in the following applications:

  • FLOW
    1 publication
  • WB
    1 publication

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD43/Sialophorin Antibody (W3/13) - BSA Free

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma. CD43 cell surface antigen, also known as leukosialin and sialophorin. CD43 is a 95kD heavily glycosylated glycoprotein expressed by all leucocytes with the exception of B lymphocytes. This product is routinely tested in flow cytometry on rat splenocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD43/Sialophorin Antibody (NB100-64992)(3)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: FLOW, WB.


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Bioinformatics

Gene Symbol SPN