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CD40/TNFRSF5 Antibody (1C10) [Unconjugated]

 (10 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IP, CyTOF-ready, Func
Clone
1C10
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB440U-100UG
Alexa Fluor 405
FAB440V-100UG
Alexa Fluor 488
FAB440G-100UG
Alexa Fluor 532
FAB440X
Alexa Fluor 594
FAB440T-100UG
Alexa Fluor 647
FAB440R-100UG
Alexa Fluor 700
FAB440N-100UG
Alexa Fluor 750
FAB440S-100UG
Allophycocyanin/Cy7
FAB440APCCY7
Biotin
BAM440
CoraFluor 1
FAB440CL1
DyLight 350
FAB440D
DyLight 405
FAB440E
DyLight 488
FAB440K
DyLight 550
FAB440L
DyLight 594
FAB440M
DyLight 650
FAB440W
DyLight 680
FAB440Y
DyLight 755
FAB440Z
Fluorescein
FAB440F
HRP
FAB440H
Janelia Fluor 525
FAB440JF525
Janelia Fluor 549
FAB440I
Janelia Fluor 646
FAB440J
Janelia Fluor 669
FAB440JF669
PE/Atto594
FAB440PEATT594
PE/Cy5.5
FAB440PECY55
PE/Cy7
FAB440PECY7
mFluor Violet 450 SE
FAB440MFV450
mFluor Violet 500 SE
FAB440MFV500
mFluor Violet 610 SE
FAB440MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB440F
Carrier Free
MAB440-SP
Carrier Free
MAB440-500
Carrier Free
FAB440V-100UG
Carrier Free
FAB440U-100UG
Carrier Free
FAB440T-100UG
Carrier Free
FAB440S-100UG
Carrier Free
FAB440R-100UG
Carrier Free
FAB440N-100UG
Carrier Free
FAB440G-100UG
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CD40/TNFRSF5 Antibody (1C10) [Unconjugated] Summary

Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse CD40/TNFRSF5
Extracellular domain
Specificity
Specifically binds mouse CD40/TNFRSF5 expressed on B cells.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD40
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Agonist Activity
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation
Publications
Read Publications using
MAB440 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD40/TNFRSF5 Antibody (1C10) [Unconjugated]

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Background

CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).

  1. Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
  2. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  3. Armitage, R.J. et al. (1993) J. Immunol. 150:3671.
  4. Callard, R.E. et al. (1993) Immunol. Today 14:559.
  5. Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487.
  6. Pullen, S.S. et al. (1999) Biochemistry 38:10168.
  7. Faris, M. et al. (1994) J. Exp. Med. 179:1923.
  8. Hanissian, S.H. and R.S. Geha (1997) Immunity 6:379.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD40/TNFRSF5 Antibody (MAB440)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Bioassay, Flow Cytometry, Functional Assay, In Vivo, Neutralization.


Filter By Application
Bioassay
(1)
Flow Cytometry
(1)
Functional Assay
(2)
In Vivo
(1)
Neutralization
(1)
All Applications
Filter By Species
Mouse
(6)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB440 Applications Species
A Ortega-Mol, C Lebrero-Fe, A Sanz, N Deleyto-Se, AB Plata-Góme, C Menéndez, O Graña-Cast, E Caleiras, A Efeyan Inhibition of Rag GTPase signaling in mice suppresses B cell responses and lymphomagenesis with minimal detrimental trade-offs Cell Reports, 2021-07-13;36(2):109372. 2021-07-13 [PMID: 34260908] (Neutralization, Mouse) Neutralization Mouse
ST Tivadar, RS McIntosh, JX Chua, R Moss, T Parsons, AM Zaitoun, S Madhusudan, LG Durrant, M Vankemmelb Monoclonal Antibody Targeting Sialyl-di-Lewisa - Containing Internalizing and non-Internalizing Glycoproteins with Cancer Immunotherapy Development Potential Mol. Cancer Ther., 2019-12-23;0(0):. 2019-12-23 [PMID: 31871270] (In Vivo, Mouse) In Vivo Mouse
Ana Ortega-Molina, Nerea Deleyto-Seldas, Joaquim Carreras, Alba Sanz, Cristina Lebrero-Fernández, Camino Menéndez et al. Oncogenic Rag GTPase signalling enhances B cell activation and drives follicular lymphoma sensitive to pharmacological inhibition of mTOR Nature Metabolism 2019-10-04 [PMID: 31579886]
Galina Obmolova, Alexey Teplyakov, Thomas J. Malia, Nicole Wunderler, Deborah Kwok, Linda Barone et al. Epitope-dependent mechanisms of CD27 neutralization revealed by X-ray crystallography Molecular Immunology 2017-03-01 [PMID: 28119207]
Sandra Santulli-Marotto, Alexis Gervais, Jamie Fisher, Brandy Strake, Carol Anne Ogden, Chelsea Riveley et al. Discovering Molecules That Regulate Efferocytosis Using Primary Human Macrophages and High Content Imaging PLOS ONE 2015-12-16 [PMID: 26674639]
Pierson E, Stromnes I, Goverman J B cells promote induction of experimental autoimmune encephalomyelitis by facilitating reactivation of T cells in the central nervous system. J Immunol, 2013-12-23;192(3):929-39. 2013-12-23 [PMID: 24367024] (Functional Assay, Mouse) Functional Assay Mouse
Matthias Hock, Martin Vaeth, Ronald Rudolf, Amiya Kumar Patra, Duong Anh Thuy Pham, Khalid Muhammad et al. NFATc1 Induction in Peripheral T and B Lymphocytes The Journal of Immunology 2013-03-01 [PMID: 23365084]
Lechouane F, Bonaud A, Delpy L, Casola S, Oruc Z, Chemin G, Cogne M, Sirac C B-cell receptor signal strength influences terminal differentiation. Eur J Immunol, 2013-01-24;43(3):619-28. 2013-01-24 [PMID: 23280426] (Bioassay, Mouse) Bioassay Mouse
Liu J, Yoshida Y, Yamashita U Suppressive effect of reactive oxygen species on CD40-induced B cell activation. FEBS Lett., 2007-09-29;581(26):5043-9. 2007-09-29 [PMID: 17919601] (Flow Cytometry, Mouse) Flow Cytometry Mouse
de la Fuente MA, Kumar L, Lu B, Geha RS 3BP2 deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation. Mol. Cell. Biol., 2006-07-01;26(14):5214-25. 2006-07-01 [PMID: 16809760] (Functional Assay, Mouse) Functional Assay Mouse

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FAQs for CD40/TNFRSF5 Antibody (MAB440). (Showing 1 - 2 of 2 FAQs).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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Bioinformatics

Gene Symbol CD40