CD31/PECAM-1 Antibody (9G11) [Unconjugated]

Images

 
Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated ...read more
CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were ...read more
CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Mouse Anti-Human ...read more
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Artery in Human Liver using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) at 10 µg/mL for 1 hour ...read more
Characterization&functional analysis of expanded AEPCs compared with HUVECs. All the experiments performed using AEPCs&HUVECs at passage number 5. (A) Fluorescent immunocytochemistry of the expression profiles of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ICC/IF, KO
Clone
9G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

CD31/PECAM-1 Antibody (9G11) [Unconjugated] Summary

Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM‑1, or recombinant mouse VCAM‑1 was observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PECAM1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Immunoprecipitation
  • Knockout Validated 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
BBA7 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody (9G11) [Unconjugated]

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • EndoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet endothelial cell adhesion molecule-1
  • platelet/endothelial cell adhesion molecule

Background

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (BBA7)(65)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Porcine, Primate - Macaca mulatta (Rhesus Macaque), Rhesus Macaque.

We have publications tested in 12 applications: Bioassay, Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(4)
Functional Assay
(1)
ICC
(15)
IF
(1)
IHC
(15)
IHC-Fr
(2)
IHC-P
(2)
Immunocytochemistry
(1)
Immunocytochemistry/ Immunofluorescence
(1)
Immunohistochemistry
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(36)
Mouse
(6)
Rat
(2)
Porcine
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rhesus Macaque
(1)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using BBA7 Applications Species
Kim, SR;Min, EK;Lee, CM;Lee, JW;Park, CH;Jung, Y;Oh, BC;Lee, HY; Development of Advanced Oral-on-a-Chip: Replicating the Intricate Human Oral Microenvironment International journal of biological sciences 2024-10-28 [PMID: 39664582] (Immunocytochemistry, Human) Immunocytochemistry Human
Vazana-Netzarim, R;Elmalem, Y;Sofer, S;Bruck, H;Danino, N;Sarig, U; Distinct HAND2/HAND2-AS1 Expression Levels May Fine-Tune Mesenchymal and Epithelial Cell Plasticity of Human Mesenchymal Stem Cells International journal of molecular sciences 2023-11-20 [PMID: 38003736] (IHC, Human) IHC Human
McQueen, LW;Ladak, SS;Layton, GR;Wadey, K;George, SJ;Angelini, GD;Murphy, GJ;Zakkar, M; Osteopontin Activation and Microcalcification in Venous Grafts Can Be Modulated by Dexamethasone Cells 2023-11-15 [PMID: 37998362] (IHC, Porcine, Human) IHC Porcine, Human
Fayed, HS;Bakleh, MZ;Ashraf, JV;Howarth, A;Ebner, D;Al Haj Zen, A; Selective ROCK Inhibitor Enhances Blood Flow Recovery after Hindlimb Ischemia International journal of molecular sciences 2023-09-22 [PMID: 37833857] (Immunocytochemistry/ Immunofluorescence, Human) Immunocytochemistry/ Immunofluorescence Human
Nakamura, Y;Shimizu, Y;Fujimaki-Shiraishi, M;Uchida, N;Takemasa, A;Niho, S; A Protective Effect of Pirfenidone in Lung Fibroblast-Endothelial Cell Network via Inhibition of Rho-Kinase Activity Biomedicines 2023-08-12 [PMID: 37626755] (ICC, Human) ICC Human
Chen, Y;Gong, C;Chauhan, S;De La Cruz, ED;Datta, MS;Tomer, R; Scalable projected Light Sheet Microscopy for high-resolution imaging of large samples bioRxiv : the preprint server for biology 2023-06-05 [PMID: 37333196] (Immunohistochemistry, Human) Immunohistochemistry Human
M Thakar, ME Noumbissi, MF Stins Microvascular Environment Influences Brain Microvascular Heterogeneity: Relative Roles of Astrocytes and Oligodendrocytes for the EPCR Expression in the Brain Endothelium International Journal of Molecular Sciences, 2023-04-07;24(8):. 2023-04-07 [PMID: 37108071] (ICC, Human) ICC Human
SR Park, MG Kook, SR Kim, JW Lee, CH Park, BC Oh, Y Jung, IS Hong Development of cell-laden multimodular Lego-like customizable endometrial tissue assembly for successful tissue regeneration Biomaterials research, 2023-04-21;27(1):33. 2023-04-21 [PMID: 37085887] (Immunohistochemistry, Human) Immunohistochemistry Human
S Johnson, Y Karpova, D Guo, A Ghatak, DA Markov, AV Tulin PARG suppresses tumorigenesis and downregulates genes controlling angiogenesis, inflammatory response, and immune cell recruitment BMC Cancer, 2022-05-18;22(1):557. 2022-05-18 [PMID: 35585513] (IHC, Mouse) IHC Mouse
DA Nawrot, LY Ozer, A Al Haj Zen A Novel High Content Angiogenesis Assay Reveals That Lacidipine, L-Type Calcium Channel Blocker, Induces In Vitro Vascular Lumen Expansion International Journal of Molecular Sciences, 2022-04-28;23(9):. 2022-04-28 [PMID: 35563280] (IHC, Human) IHC Human
Show All 65 Publications.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Additional CD31/PECAM-1 Products

Blogs on CD31/PECAM-1.


  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Bioinformatics

Gene Symbol PECAM1