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CD3 Antibody (17A2) [Alexa Fluor® 647]

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Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
17A2
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 647

Order Details

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CD3 Antibody (17A2) [Alexa Fluor® 647] Summary

Immunogen
T cell hybridoma D1
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CD3E
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 3
using
FAB4841R in the following application:

Publications
Read Publication using
FAB4841R in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 Antibody (17A2) [Alexa Fluor® 647]

  • CD_antigen: CD3e
  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • IMD18
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma. This antibody has been shown to react with the epsilon chain of the CD3 complex. The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 Antibody (FAB4841R)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Immunohistochemistry.


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Review for CD3 Antibody (FAB4841R) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using FAB4841R:
Filter by Applications
Flow
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CD3 FAB4841R
Enlarge
3
reviewed by:
Li Chou
Flow Mouse 01/30/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedMurine Splenocytes (C57B6)
SpeciesMouse
LotAEIY0114122

Comments

CommentsC576BL/6 splenocytes were isolated and stained.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD3 Antibody (FAB4841R) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Cluster of Differentiation 3 (CD3) (OKT3 clone) as a Marker of Immune Response Efficiency
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The CD4 Antibody: More than Just a Cellular Marker
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Recent Reviews

3
5
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4
0
3
1
2
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1
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Li Chou
01/30/2019
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol CD3E