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CD24 Antibody

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Immunocytochemistry/ Immunofluorescence: CD24 Antibody [NBP1-46390] - Mouse WAT-CM induces the expression of CD24 in mesenchymal MDA-MB-231 cells but does not affect the expression of vimentin. Representative images of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD24 Antibody Summary

Immunogen
A synthetic peptide from aa region 30-70 of human mature Signal transducer CD24 conjugated to blue carrier protein was used as the antigen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD24
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1:500-1:1000
Application Notes
The optimal dilution should be determined by the end user. Not tested in other applications. ICC/IF was reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 31040372).
Publications
Read Publications using
NBP1-46390 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23301003).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from whole antisera
Preservative
No Preservative
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for CD24 Antibody

  • CD 24
  • CD24 antigen (small cell lung carcinoma cluster 4 antigen)
  • CD24 antigen
  • CD24 molecule
  • CD24
  • CD24A
  • CD24Asignal transducer CD24
  • FLJ22950
  • FLJ43543
  • MGC75043
  • Small cell lung carcinoma cluster 4 antigen

Background

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD24 Antibody (NBP1-46390)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: FLOW, ICC/IF, IF/IHC, Immunohistochemistry, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IF/IHC
(3)
Immunohistochemistry
(1)
WB
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-46390 Applications Species
Ilie DS, Mitroi G, P?un I et al. Pathological and immunohistochemical study of colon cancer. Evaluation of markers for colon cancer stem cells Romanian Journal of Morphology and Embryology 2021-10-10 [PMID: 34609414] (Immunohistochemistry) Immunohistochemistry
Asante, E C, Pallegar, N K Et al. Adipose Tissue from Lean and Obese Mice Induces a Mesenchymal to Epithelial Transition-Like Effect in Triple Negative Breast Cancers Cells Grown in 3-Dimensional Culture. Int J Mol Sci 2020-09-03 [PMID: 32899433] (WB, Human) WB Human
Sun H, Yao N, Cheng S et al. Cancer stem-like cells directly participate in vasculogenic mimicry channels in triple-negative breast cancer Cancer Biol Med 2019-05-01 [PMID: 31516750] (IF/IHC, Human) IF/IHC Human
Ren G, Zheng X, et al. Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGF beta. Sci Rep 2019-04-30 [PMID: 31040372] (IF/IHC, FLOW, Human) IF/IHC, FLOW Human
Barash U, Spyrou A, Liu P, Vlodavsky E et al. Heparanase promotes glioma progression via enhancing CD24 expression Int. J. Cancer 2019-04-29 [PMID: 31032901]
Pallegar NK, Garland CJ, Mahendralingam M et al. A Novel 3-Dimensional Co-culture Method Reveals a Partial Mesenchymal to Epithelial Transition in Breast Cancer Cells Induced by Adipocytes. J Mammary Gland Biol Neoplasia. 2018-11-24 [PMID: 30474817] (Human) Human
Choi Y, Kim HS, Cho KW et al. Noninvasive identification of viable cell populations in docetaxel-treated breast tumors using ferritin-based magnetic resonance imaging PLoS One 2013-01-01 [PMID: 23301003] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD24 Antibody (NBP1-46390) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD24
Entrez
Uniprot