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CD1a Antibody (O10) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10).
Flow Cytometry: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697] - Flow Cytometric Analysis of MOLT-4 cells using CD1a Antibody (O10) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697] - Human skin. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
O10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

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CD1a Antibody (O10) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34313).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Clone O10 was used by HLDA to establish CD designation.
Immunogen
Human thymus cells
Localization
Cell surface
Marker
Mature Langerhans Cells Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot 0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34697 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CD1a Antibody (O10) - Azide and BSA Free

  • CD1
  • CD1a antigen
  • CD1A antigen, a polypeptide
  • CD1a molecule
  • CD1a
  • cluster of differentiation 1 A
  • cortical thymocyte antigen CD1A
  • differentiation antigen CD1-alpha-3
  • epidermal dendritic cell marker CD1a
  • FCB6
  • hTa1 thymocyte antigen
  • HTA1
  • R4
  • T6
  • T-cell surface antigen T6/Leu-6
  • T-cell surface glycoprotein CD1a

Background

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD1a Antibody (NBP2-34697) (0)

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Review for CD1a Antibody (NBP2-34697) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34697:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD1a NBP2-34697
Enlarge
5
reviewed by:
Santhosh Sivajothi
IHC-P Human 05/21/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman skin
SpeciesHuman
Lot909-IPABX170320

Comments

CommentsHeat induced antigen retrieval was performed in citrate buffer (pH 6) at 95C for 20 minutes

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD1a Antibody (NBP2-34697) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
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2
0
1
0

Santhosh Sivajothi
05/21/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CD1A