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CD117/c-kit Antibody (180627) [DyLight 550]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
180627
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1356U-100UG
Alexa Fluor 405
FAB1356V-100UG
Alexa Fluor 488
FAB1356G-100UG
Alexa Fluor 532
FAB1356X
Alexa Fluor 594
FAB1356T-100UG
Alexa Fluor 647
FAB1356R-100UG
Alexa Fluor 700
FAB1356N-100UG
Alexa Fluor 750
FAB1356S-100UG
Allophycocyanin
FAB1356A
Allophycocyanin/Cy7
FAB1356APCCY7
Biotin
BAM1356
DyLight 350
FAB1356D
DyLight 405
FAB1356E
DyLight 488
FAB1356K
DyLight 594
FAB1356M
DyLight 650
FAB1356W
DyLight 680
FAB1356Y
DyLight 755
FAB1356Z
FITC
FAB1356F
HRP
FAB1356H
Janelia Fluor 549
FAB1356I
Janelia Fluor 646
FAB1356J
PE/Atto594
FAB1356PEATT594
PE/Cy5.5
FAB1356PECY55
PE/Cy7
FAB1356PECY7
PerCP
FAB1356C
Phycoerythrin
FAB1356P-025
Phycoerythrin
FAB1356P-100
Unconjugated
MAB1356-100
mFluor Violet 450 SE
FAB1356MFV450
mFluor Violet 500 SE
FAB1356MFV500
mFluor Violet 610 SE
FAB1356MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1356-100
Carrier Free
FAB1356A
Carrier Free
MAB1356-SP
Carrier Free
MAB1356-500
Carrier Free
MAB1356-100
Carrier Free
FAB1356P-100
Carrier Free
FAB1356P-025
Carrier Free
FAB1356V-100UG
Carrier Free
FAB1356U-100UG
Carrier Free
FAB1356T-100UG
Carrier Free
FAB1356S-100UG
Carrier Free
FAB1356R-100UG
Carrier Free
FAB1356N-100UG
Carrier Free
FAB1356G-100UG
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CD117/c-kit Antibody (180627) [DyLight 550] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Specificity
Detects mouse CD117/c-kit in direct ELISAs.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
KIT
Purity
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD117/c-kit Antibody (180627) [DyLight 550]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Bioinformatics

Gene Symbol KIT