Home » CCL28 » CCL28 Antibodies » CCL28 Antibody
Novus Biologicals products are now on bio-techne.com

CCL28 Antibody

 (5 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF533). The ND50 is typically 5-25 µg/mL.
Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF533
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF533-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CCL28 Antibody Summary

Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Specificity
Detects mouse CCL28 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human CCL28 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL28
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 5-25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF533 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL28 Antibody

  • CCL28
  • chemokine (C-C motif) ligand 28
  • MEC
  • member 28
  • MGC71902
  • Protein CCK1
  • VIC

Background

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Cholecystokinin
Antibody
NBP3-18225
Immunocytochemistry/ Immunofluorescence Cholecystokinin Antibody
Immunohistochemistry-Paraffin Cholecystokinin Antibody
Western Blot Cholecystokinin Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-P, WB
RIPK2 Antibody
(6F7)
H00008767-M02
ELISA RIPK2 Antibody (6F7)
Immunohistochemistry-Paraffin RIPK2 Antibody (6F7)
Western Blot RIPK2 Antibody (6F7)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
Cholecystokinin-B
R/CCKBR Antibody
NB100-2803
Western Blot Cholecystokinin-B R/CCKBR Antibody
Immunohistochemistry Cholecystokinin-B R/CCKBR Antibody
Immunohistochemistry Cholecystokinin-B R/CCKBR Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
CC Chemokine
Receptor D6 Antibody ...
MAB1364
Flow Cytometry CC Chemokine Receptor D6 Antibody (196124) [Unconjugated]
(5 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
CCL25/TECK
[Unconjugated]
481-TK
CCL25/TECK [Unconjugated]
(17 Publications)
Species: Mu
Applications: BA
CCK-AR Antibody
[Unconjugated]
AF2680
Immunohistochemistry CCK-AR Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, WB
CCR10/GPR2 Antibody
NB100-56319
Simple Western CCR10/GPR2 Antibody
Western Blot CCR10/GPR2 Antibody
(6 Publications)
Species: Hu
Applications: Flow-CS, Flow-IC, Flow, IHC, IHC-Fr, Simple Western, WB
CCR11 Antibody
(674144) [Unconjugate
MAB6899
Flow Cytometry CCR11 Antibody (674144) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, Flow
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(854 Publications)
Species: Hu
Applications: BA
CCL27/CTACK [HRP]
DCC270
N/A CCL27/CTACK [HRP]
N/A CCL27/CTACK [HRP]
(10 Publications)
Species: Hu
Applications: ELISA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(670 Publications)
Species: Mu
Applications: ELISA
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(184 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
CCR9 Antibody
(112509) [Unconjugate
MAB179
Immunocytochemistry CCR9 Antibody (112509) [Unconjugated]
(11 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC
IGF-I/IGF-1
[Unconjugated]
291-G1
SDS-PAGE IGF-I/IGF-1 [Unconjugated]
Bioactivity IGF-I/IGF-1 [Unconjugated]
Mass Spectrometry IGF-I/IGF-1 [Unconjugated]
(224 Publications)
Species: Hu
Applications: BA
Gastrin Antibody
NBP3-12223
Immunohistochemistry-Paraffin Gastrin Antibody
Unknown Gastrin Antibody
Species: Hu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
CCR3 Antibody
(61828)
MAB155
Immunohistochemistry CCR3 Antibody (61828)
Neutralization CCR3 Antibody (61828)
(20 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
CXCL12/SDF-1 alpha
[Unconjugated]
350-NS
SDS-PAGE CXCL12/SDF-1 alpha [Unconjugated]
Bioactivity CXCL12/SDF-1 alpha [Unconjugated]
(189 Publications)
Species: Fe, Hu, RM
Applications: BA, BA

Publications for CCL28 Antibody (AF533)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC-Fr, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC-Fr
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF533 Applications Species
Cha HR, Ko HJ, Kim ED Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. J. Immunol., 2011-08-10;187(6):3044-52. 2011-08-10 [PMID: 21832166] (Western Blot, Mouse) Western Blot Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination. J. Immunol., 2009-06-01;182(11):6851-60. 2009-06-01 [PMID: 19454681] (Neutralization, Mouse) Neutralization Mouse
Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells. J. Immunol., 2004-09-15;173(6):3668-75. 2004-09-15 [PMID: 15356112] (Neutralization, Mouse) Neutralization Mouse
Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. J. Immunol., 2003-02-01;170(3):1452-61. 2003-02-01 [PMID: 12538707] (IHC-Fr, Mouse) IHC-Fr Mouse

Reviews for CCL28 Antibody (AF533) (0)

There are no reviews for CCL28 Antibody (AF533). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL28 Antibody (AF533) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CCL28 Products

Blogs on CCL28

There are no specific blogs for CCL28, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCL28 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol CCL28
Uniprot