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CCL27/CTACK Antibody (124308) [Unconjugated]

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Recombinant Human CCL27/CTACK (Catalog #376‑CT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL27/CTACK Monoclonal Antibody (Catalog # MAB376). The ND50 is typically0.5-3.0 µg/mL.
Recombinant Human CCL27/CTACK (Catalog #376‑CT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more
CCL27/CTACK was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CCL27/CTACK Monoclonal Antibody (Catalog # MAB376) at 25 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clone
124308
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB376-SP
Carrier Free
MAB376-500
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CCL27/CTACK Antibody (124308) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL27/CTACK
Phe25-Gly112
Accession # NP_006655
Specificity
Detects human CCL27/CTACK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL27
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.5-3.0 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB376 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL27/CTACK Antibody (124308) [Unconjugated]

  • ALP
  • C-C motif chemokine 27
  • CCL27
  • chemokine (C-C motif) ligand 27
  • CTACK
  • CTACKSmall-inducible cytokine A27
  • CTAK
  • cutaneous T-cell attracting chemokine
  • Cutaneous T-cell-attracting chemokine
  • ESKINE
  • IL-11 Ralpha-locus chemokine
  • ILC
  • ILCCC chemokine ILC
  • PESKY
  • SCYA27IL-11 R-alpha-locus chemokine
  • skinkine
  • small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11%‑35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is up‑regulated by inflammatory stimuli and in wounded skin (4‑7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10‑12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13‑15).

  1. Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
  2. Ishikawa-Mochizuki, I. et al. (1999) FEBS Lett. 460:544.
  3. Gortz, A. et al. (2002) J. Immunol. 169:1387.
  4. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.
  5. Homey, B. et al. (2000) J. Immunol. 164:3465.
  6. Homey, B. et al. (2002) Nat. Med. 8:157.
  7. Inokuma, D. et al. (2006) Stem Cells 24:2810.
  8. Jarmin, D. et al. (2000) J. Immunol. 164:3460.
  9. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
  10. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
  11. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
  12. Soler, D. et al. (2003) Blood 101:1677.
  13. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
  14. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
  15. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL27/CTACK
Antibody (124308) [Un ...
MAB376
CCL27/CTACK Antibody (124308) [Unconjugated]
Species: Hu
Applications: WB, IHC, B/N

Publications for CCL27/CTACK Antibody (MAB376)(6)

We have publications tested in 2 confirmed species: Human, Xenograft.

We have publications tested in 4 applications: Granzyme B Activity, ICC, IHC-P, Neutralization.


Filter By Application
Granzyme B Activity
(1)
ICC
(1)
IHC-P
(3)
Neutralization
(1)
All Applications
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Human
(5)
Xenograft
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB376 Applications Species
T Karnezis, R Farnsworth, NC Harris, SP Williams, C Caesar, DJ Byrne, P Herle, ML Macheda, R Shayan, YF Zhang, S Yazar, SJ Takouridis, C Gerard, S Fox, MG Achen, SA Stacker CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS Cancer Res., 2019-02-01;0(0):. 2019-02-01 [PMID: 30709930] (IHC-P, Neutralization, Human, Xenograft) IHC-P, Neutralization Human, Xenograft
XW Yang, HX Jiang, R Lei, WS Lu, SH Tan, SY Qin Recruitment and significance of Th22 cells and Th17 cells in malignant ascites Oncol Lett, 2018-08-16;16(4):5389-5397. 8/16/2018 [PMID: 30250609]
Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Granzyme B Activity, Human) Granzyme B Activity Human
Goteri G, Rupoli S, Campanati A, Zizzi A, Picardi P, Cardelli M, Giantomassi F, Canafoglia L, Marchegiani F, Mozzicafreddo G, Brandozzi G, Stramazzotti D, Ganzetti G, Lisa R, Simonetti O, Offidani A, Federici I, Filosa G, Leoni P Serum and tissue CTACK/CCL27 chemokine levels in early mycosis fungoides may be correlated with disease-free survival following treatment with interferon alfa and psoralen plus ultraviolet A therapy. Br. J. Dermatol., 2012-05-01;166(5):948-52. 2012-05-01 [PMID: 22233400] (IHC-P, Human) IHC-P Human
Kaporis HG, Guttman-Yassky E, Lowes MA, Haider AS, Fuentes-Duculan J, Darabi K, Whynot-Ertelt J, Khatcherian A, Cardinale I, Novitskaya I, Krueger JG, Carucci JA Human basal cell carcinoma is associated with Foxp3+ T cells in a Th2 dominant microenvironment. J. Invest. Dermatol., 2007-05-17;127(10):2391-8. 2007-05-17 [PMID: 17508019] (ICC, Human) ICC Human
Sasaki M, Honda A, Furuichi M, Norisugi O Presence of circulating CCR10+ T cells and elevated serum CTACK/CCL27 in the early stage of mycosis fungoides. Clin. Cancer Res., 2006-05-01;12(9):2670-5. 2006-05-01 [PMID: 16675558] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol CCL27
Uniprot