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Recombinant Rabbit CCL2/MCP1 Protein

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA
Concentration
LYOPH

Order Details

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Recombinant Rabbit CCL2/MCP1 Protein Summary

Description
Recombinant rabbit MCP-1.

Source:Pichia pastoris

Amino Acid Sequence:aa 24 - 125 of native rabbit monocyte chemotactic protein 1 (MCP-1), UniProt P28292

Preparation
Method
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
Source
Pichia pastoris
Protein/Peptide Type
Recombinant Protein
Gene
CCL2
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Theoretical MW
11.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
10% Trehalose
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.

Alternate Names for Recombinant Rabbit CCL2/MCP1 Protein

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

Monocyte Chemoattracctant Protein-1 (MCP1), also known as CCL2, is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli and is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. MCP1 augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. In atherosclerosis MCP-1 has been implicated to be involved in the recruitment of monocytes into the arterial wall during the disease process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Research Areas for CCL2/JE/MCP-1 Recombinant Protein (NBP2-62500)

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Blogs on CCL2/MCP1.

MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

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Bioinformatics

Gene Symbol CCL2
Uniprot