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Mouse CCL2/MCP1 ELISA Kit (Colorimetric)

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Mouse CCL2/MCP1 ELISA Kit [NBP1-92659] - Data for Mouse MCP-1 Platinum ELISA
Mouse CCL2/MCP1 ELISA Kit [NBP1-92659] - Data for Mouse MCP-1 Platinum ELISA

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
2.2 pg/mL

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Mouse CCL2/MCP1 ELISA Kit (Colorimetric) Summary

Description
The mouse MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse MCP-1
Specificity
The assay detects both natural and recombinant mouse MCP-1. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse MCP-1 positive serum. There was no crossreactivity detected, notably not with TCA3 and MIP-1 beta.
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
2.2 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 5.60%
Intra-Assay
CV% < 6.20%
Spike Recovery
The recovery ranged from 80% to 111% with an overall mean recovery of 98%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CCL2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse MCP-1
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-mouse MCP-1 monoclonal antibody
  5. Mouse MCP-1 Standard lyophilized, 2 ng/ml upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Mouse CCL2/MCP1 ELISA Kit (Colorimetric)

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

MCP-1was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-alpha and IL-1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells. Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Manual - Mouse CCL2/MCP1 ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol CCL2