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Human CCL2/MCP1 ELISA Kit (Colorimetric)

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ELISA: CCL2/MCP1 ELISA Kit [NBP1-91276] - Standard curve
ELISA: CCL2/MCP1 ELISA Kit [NBP1-91276] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
2.3 pg/mL

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Human CCL2/MCP1 ELISA Kit (Colorimetric) Summary

Description
The human MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human MCP-1.
Specificity
The assay detects both natural and recombinant human MCP-1. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human MCP- 1 positive serum. There was no cross reactivity detected, namely not with MCP-3.
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
2.3 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 8.70%
Intra-Assay
CV% < 4.70%
Spike Recovery
The recovery ranged from 84% to 98% with an overall mean recovery of 92%. Please see protocol for more specific Spike Recovery details.
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CCL2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a human MCP-1 Standard curve (coloured)
  3. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MCP-1, HRP-Conjugate (human MCP-1 monoclonal antibody) and Assay Buffer, lyophilized
  4. Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
  5. Stop Solution (1M Phosphoric acid)
  6. Substrate Solution (tetramethyl-benzidine)
  7. Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Alternate Names for Human CCL2/MCP1 ELISA Kit (Colorimetric)

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

MCP-1was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells. The human mature MCP-1 protein consists of 76 amino acids, derived by cleavage of a hydrophobic signal peptide from the 99 aa precursor protein. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-alpha and IL-1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells. Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes, and expression of the MCP-1 chemokine may affect HIV infection via signaling through the CCR2 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Manual - Human CCL2/MCP1 ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol CCL2