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CCL2/MCP1 Antibody (2D8) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Fe, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
2D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22115AF350
Alexa Fluor 405
NBP2-22115AF405
Alexa Fluor 488
NBP2-22115AF488
Alexa Fluor 532
NBP2-22115AF532
Alexa Fluor 594
NBP2-22115AF594
Alexa Fluor 647
NBP2-22115AF647
Alexa Fluor 700
NBP2-22115AF700
Alexa Fluor 750
NBP2-22115AF750
Allophycocyanin
NBP2-22115APC
Biotin
NBP2-22115B
CoraFluor 1
NBP2-22115CL1
DyLight 350
NBP2-22115UV
DyLight 405
NBP2-22115V
DyLight 488
NBP2-22115G
DyLight 550
NBP2-22115R
DyLight 594
NBP2-22115DL594
DyLight 650
NBP2-22115C
DyLight 680
NBP2-22115FR
DyLight 755
NBP2-22115IR
FITC
NBP2-22115F
HRP
NBP2-22115H
Janelia Fluor 525
NBP2-22115JF525
Janelia Fluor 549
NBP2-22115JF549
Janelia Fluor 585
NBP2-22115JF585
Janelia Fluor 646
NBP2-22115JF646
Janelia Fluor 669
NBP2-22115JF669
PE
NBP2-22115PE
PerCP
NBP2-22115PCP
Unconjugated
NBP2-22115
mFluor Violet 450 SE
NBP2-22115MFV450
mFluor Violet 500 SE
NBP2-22115MFV500
mFluor Violet 610 SE
NBP2-22115MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22115
BSA Free
NBP2-22115
BSA Free
NBP2-22115SS
Azide and BSA Free
NBP2-80618
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CCL2/MCP1 Antibody (2D8) [Janelia Fluor® 635] Summary

Immunogen
Full lenght human MCP1 expressed in E. coli. [Uniprot: P13500]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL2
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29286133).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CCL2/MCP1 Antibody (2D8) [Janelia Fluor® 635]

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

Monocyte Chemoattracctant Protein-1 (MCP1), also known as CCL2, is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli and is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. MCP1 augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. In atherosclerosis MCP-1 has been implicated to be involved in the recruitment of monocytes into the arterial wall during the disease process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: CyTOF-ready, ICC, IHC, ICFlow

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Video Protocols

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Secondary Antibodies

 

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MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.
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Bioinformatics

Gene Symbol CCL2