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CBL Antibody (6D12)

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Western Blot: CBL Antibody (6D12) [H00000867-M01] - Analysis of CBL expression in transfected 293T cell line by CBL monoclonal antibody (M01), clone 6D12.Lane 1: CBL transfected lysate (Predicted MW: 99.6 KDa).Lane 2: Non-transfected lysate.
Western Blot: CBL Antibody (6D12) [H00000867-M01] - Analysis of CBL expression in transfected 293T cell line by CBL monoclonal antibody (M01), clone 6D12.Lane 1: CBL transfected lysate (Predicted MW: 99.6 KDa).Lane 2: ...read more
Western Blot: CBL Antibody (6D12) [H00000867-M01] - CBL monoclonal antibody (M01), clone 6D12 Analysis of CBL expression in K-562.
Immunoprecipitation: CBL Antibody (6D12) [H00000867-M01] - Analysis of CBL transfected lysate using anti-CBL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CBL monoclonal antibody.
Sandwich ELISA: CBL Antibody (6D12) [H00000867-M01] - Detection limit for recombinant GST tagged CBL is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
6D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CBL Antibody (6D12) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CBL (NP_005179, 736 a.a. ~ 835 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSER
Specificity
CBL - Cas-Br-M (murine) ecotropic retroviral transforming sequence
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CBL
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CBL Antibody (6D12)

  • Cas-Br-M (murine) ecotropic retroviral transforming sequence
  • Casitas B-lineage lymphoma proto-oncogene
  • CBL
  • CBL2
  • CBL2E3 ubiquitin-protein ligase CBL
  • EC 6.3.2
  • EC 6.3.2.-
  • oncogene CBL2
  • Proto-oncogene c-Cbl
  • RING finger protein 55
  • RNF55
  • RNF55C-CBL
  • Signal transduction protein CBL

Background

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00000867-M01
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CBL
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