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Caveolin-1 Antibody

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Western Blot: Caveolin-1 Antibody [NBP2-54737] - Analysis in human cell line A-431 and human cell line CACO-2.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Analysis in human lung and pancreas tissues. Corresponding CAV1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human liver shows moderate membranous positivity in hepatic sinusoid cells.
Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human placenta shows strong membranous positivity in endothelial cells.
Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human lung shows strong membranous positivity in pneumocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Caveolin-1 Antibody Summary

Immunogen
This antibody was developed against a Recombinant Protein corresponding to amino acids: LIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAV1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Caveolin-1 Recombinant Protein Antigen (NBP2-54737PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Caveolin-1 Antibody

  • CAV
  • CAV1
  • caveolae protein, 22kD
  • caveolin 1, caveolae protein, 22kDa
  • Caveolin1
  • Caveolin-1
  • cell growth-inhibiting protein 32
  • MSTP085
  • VIP21

Background

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CAV1