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CARS Antibody (CL2302)

Images

 
Western Blot: CARS Antibody (CL2302) [NBP2-36755] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear ...read more
Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755] - Staining of human skeletal muscle shows absence of immunoreacitivity.
Western Blot: CARS Antibody (CL2302) [NBP2-36755] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755] - Staining of human breast cancer shows cytoplasmic immunoreactivity in tumor cells.
Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in glandular epithelium cells.
Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL2302
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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CARS Antibody (CL2302) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE
Epitope
RPNQALLENIALYLT
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CARS1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CARS Recombinant Protein Antigen (NBP2-36755PEP)

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (86%), Rat (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for CARS Antibody (CL2302)

  • CARS1
  • CysRS
  • cysteine translase
  • cysteine tRNA ligase 1, cytoplasmic
  • Cysteine--tRNA ligase
  • cysteinyl-tRNA synthetase
  • cysteinyl-tRNA synthetase, cytoplasmic
  • EC 6.1.1
  • EC 6.1.1.16
  • MGC:11246

Background

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CARS1
Uniprot