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Recombinant Rat Cardiotrophin-1/CT-1 Protein

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SDS-Page: Rat Cardiotrophin-1/CT-1 Protein [NBP2-35292]
SDS-Page: Rat Cardiotrophin-1/CT-1 Protein [NBP2-35292]

Product Details

Summary
Reactivity RtSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35292-10ug
Carrier-Free
NBP2-35292-1mg
Carrier-Free
NBP2-35292-500ug
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Recombinant Rat Cardiotrophin-1/CT-1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 203 amino acids corresponding to Cardiotrophin-1/CT-1 Source: E. coli

Uniprot ID: Q63086

Amino Acid Sequence: MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Details of Functionality
Cardiotrophin 1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CTF1
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of Cardiotrophin-1/CT-1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Rat Cardiotrophin-1/CT-1 Protein

  • cardiophin 1
  • cardiotrophin 1
  • Cardiotrophin1
  • Cardiotrophin-1
  • CT-1CT1
  • CTF1

Background

Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Rat
Cardiotrophin-1/CT-1 ...
NBP2-35292
Recombinant Rat Cardiotrophin-1/CT-1 Protein
Species: Rt
Applications: PAGE, Bioactivity

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Bioinformatics

Gene Symbol CTF1