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Recombinant Human Cardiotrophin-1/CT-1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Human Cardiotrophin-1/CT-1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 201 amino acids corresponding to Cardiotrophin-1/CT-1 Source: E. coli

Uniprot ID: Q16619

Amino Acid Sequence: MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A
Details of Functionality
Cardiotrophin-1/CT-1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0x 10^6 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CTF1
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of Cardiotrophin-1/CT-1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human Cardiotrophin-1/CT-1 Protein

  • cardiophin 1
  • cardiotrophin 1
  • Cardiotrophin1
  • Cardiotrophin-1
  • CT-1CT1
  • CTF1

Background

Cardiotrophin 1 is encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
Cardiotrophin-1/CT-1 ...
NBP2-61364
Recombinant Human Cardiotrophin-1/CT-1 Protein
Species: Hu
Applications: Bioactivity

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Bioinformatics

Gene Symbol CTF1