Carbonic Anhydrase I/CA1 Antibody (363121) [Unconjugated] Summary
Immunogen |
E. coli-derived recombinant human Carbonic Anhydrase I/CA1 Ala2-Phe261 Accession # P00915 |
Specificity |
Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) CA2 is observed and no cross-reactivity with rhCA3, -4, -7, -8, -10, -11, -12, -13, or -14 is observed. |
Source |
N/A |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Rat |
Gene |
CA1 |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Carbonic Anhydrase I/CA1 Antibody (363121) [Unconjugated]
Background
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
- Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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