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Calpain 2 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.
Western Blot: Calpain 2 Antibody [NBP1-88204] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: Calpain 2 Antibody [NBP1-88204] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human lung shows weak to moderate cytoplasmic positivity in pneumocytes.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human cervix, uterine shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli and in distal tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Calpain 2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Calpain 2 Protein (NBP1-88204PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Calpain 2 Antibody

  • Calcium-activated neutral proteinase 2
  • calpain 2, (m/II) large subunit
  • calpain 2, large [catalytic] subunit
  • calpain 2, large subunit
  • Calpain large polypeptide L2
  • Calpain M-type
  • calpain, large polypeptide L2
  • calpain-2 catalytic subunit
  • Calpain-2 large subunit
  • CANP 2
  • CANPL2CANP2
  • CANPml
  • EC 3.4.22
  • EC 3.4.22.53
  • FLJ39928
  • M-calpain
  • mCANP
  • Millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CAPN2