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Caldesmon/CALD1 Antibody (767001) [Unconjugated]

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Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caldesmon/CALD1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) followed by HRP-conjugated ...read more
Caldesmon/CALD1 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of endometrial carcinoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. ...read more
Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of melanoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
767001
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7569-SP
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Caldesmon/CALD1 Antibody (767001) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Caldesmon/CALD1
Lys696-Val793
Accession # Q05682
Specificity
Detects human Caldesmon/CALD1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CALD1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using MAB7569.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caldesmon/CALD1 Antibody (767001) [Unconjugated]

  • CAD
  • CALD1
  • caldesmon 1
  • Caldesmon
  • CDM
  • CDMH-CAD
  • HCAD
  • LCAD
  • L-CAD
  • MGC21352
  • NAG22

Background

Caldesmon, also known as CaD or h-CaD, is a 120-150 kDa cytosolic protein that is important in actin cytoskeleton dynamics. Smooth muscle Caldesmon regulates calcium-dependent smooth muscle contraction by stabilizing actin filaments, inhibiting the actomyosin ATPase, and interacting with F-actin, myosin, tropomyosin, and calmodulin. It is also a key molecule in other related actin-related processes including cytokinesis, cell migration, wound healing, and exocytosis. Caldesmon activity is regulated by serine and tyrosine phosphorylation by multiple kinases. Alternate splicing generates 70-80 kDa isoforms of human Caldesmon, known as I-CaD, that lack the central repeating region of the protein (aa 208-436 or aa 208-462). These isoforms are widely expressed and are comparable in size to full length mouse and rat Caldesmon. Within aa 696-793, human Caldesmon shares 94% and 95% aa sequence identity with mouse and rat Caldesmon, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Caldesmon/CALD1
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MAB7569
Caldesmon/CALD1 Antibody (767001) [Unconjugated]
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Publications for Caldesmon/CALD1 Antibody (MAB7569)(2)


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Bioinformatics

Gene Symbol CALD1
Uniprot