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C5L2/GPR77 Antibody

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Western Blot: C5L2/GPR77 Antibody [NBP1-86777] - Analysis in control (vector only transfected HEK293T lysate) and C5AR2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: C5L2/GPR77 Antibody [NBP1-86777] - Analysis in control (vector only transfected HEK293T lysate) and C5AR2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: C5L2/GPR77 Antibody [NBP1-86777] - Staining of human small intestine shows distinct positivity in subset of glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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C5L2/GPR77 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NDSVSYEYGDYSDLSDRPVDCLDGACLAIDPLR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
C5AR2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
C5L2/GPR77 Protein (NBP1-86777PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for C5L2/GPR77 Antibody

  • C5a anaphylatoxin chemotactic receptor C5L2
  • C5L2
  • C5L2G-protein coupled receptor 77
  • G protein-coupled receptor 77
  • G protein-coupled receptor C5L2
  • GPF77
  • GPR77

Background

Official Gene Symbol: GPR77 Gen Bank Accession Number: NP_060955 Gene ID: 27202 (human) Gene Map Locus: 19q13.33 (human) C5L2 is 337A.A orphan member that belongs to anaphylatoxin receptor family. It is a seven transmembrane G-protein-coupled receptor consisting of an N-terminal N-glycosylation site, several conserved cysteines and several potential C-terminal phosphorylation sites. It binds to C5a and C5a-des-Arg and serves as a highly regulated scavenger receptor with a negative modulatory influence on C5aR-mediated inflammation. Northern Blot analysis suggests it is co- expressed along with C5aR in different tissues and cells including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol C5AR2