![Western Blot: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa](http://images.novusbio.com/fullsize/C4-4A-LYPD3-Antibody-213-Western-Blot-NBP2-89995-img0003.jpg "Western Blot: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 213 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human C4.4A/LYPD3 (Accession#: NP_055215.2; Met 1-His 286). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LYPD3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LYPD3 |
![Immunocytochemistry/Immunofluorescence: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/C4-4A-LYPD3-Antibody-213-Immunocytochemistry-Immunofluorescence-NBP2-89995-img0001.jpg "Immunocytochemistry/Immunofluorescence: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
![Flow Cytometry: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Analysis of Human LYPD3 expression on PC-3 cells. Cells were stained with purified anti-Human LYPD3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/C4-4A-LYPD3-Antibody-213-Flow-Cytometry-NBP2-89995-img0002.jpg "Flow Cytometry: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Analysis of Human LYPD3 expression on PC-3 cells. Cells were stained with purified anti-Human LYPD3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
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