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![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human liver.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0017.jpg "Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human liver.")
![Immunohistochemistry: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human oral mucosa shows strong cytoplasmic and membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-NBP2-32598-img0010.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0011.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining in human esophagus and skeletal muscle tissues using anti-LYPD3 antibody. Corresponding LYPD3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human colon, kidney, liver and skin using Anti-LYPD3 antibody NBP2-32598 (A) shows similar protein distribution across tissues to independent antibody NBP2-32605 (B).](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0014.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human kidney.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0015.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human skin.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0016.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32598] - Staining of human colon.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32598-img0018.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32605] - Analysis in human skin and cerebral cortex tissues using NBP2-32605 antibody. Corresponding LYPD3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32605-img0002.jpg "<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32605] - Analysis in human skin and cerebral cortex tissues using NBP2-32605 antibody. Corresponding LYPD3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32605] - Staining of human cerebral cortex, kidney, liver and skin using Anti-LYPDS andibody NBP2-32605 (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32605-img0004.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32605] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32605-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32605] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32605-img0005.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32605] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32605-img0006.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP2-32605] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP2-32605-img0007.jpg)
![Western Blot: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa](http://images.novusbio.com/fullsize/C4-4A-LYPD3-Antibody-213-Western-Blot-NBP2-89995-img0003.jpg "Western Blot: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa")
![Immunocytochemistry/Immunofluorescence: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/C4-4A-LYPD3-Antibody-213-Immunocytochemistry-Immunofluorescence-NBP2-89995-img0001.jpg)
![Flow Cytometry: C4.4A/LYPD3 Antibody (213) [NBP2-89995] - Analysis of Human LYPD3 expression on PC-3 cells. Cells were stained with purified anti-Human LYPD3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/C4-4A-LYPD3-Antibody-213-Flow-Cytometry-NBP2-89995-img0002.jpg)
![Western Blot: C4.4A/LYPD3 Antibody (PS00-59) [NBP3-32550] - Western blot analysis of C4.4A/LYPD3 on different lysates with Rabbit anti-C4.4A/LYPD3 antibody (NBP3-32550) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF-7 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 90 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32550) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32550_rabbit-c4-4a-lypd3-mab-ps00-59-278202416331230.jpg "Western Blot: C4.4A/LYPD3 Antibody (PS00-59) [NBP3-32550] - Western blot analysis of C4.4A/LYPD3 on different lysates with Rabbit anti-C4.4A/LYPD3 antibody (NBP3-32550) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF-7 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 90 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32550) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: C4.4A/LYPD3 Antibody (PS00-59) [NBP3-32550] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-C4.4A/LYPD3 antibody (NBP3-32550) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32550) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32550_rabbit-c4-4a-lypd3-mab-ps00-59-2982024142098.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP1-44371] - Analysis of anti-LYPD3 antibody with human adrenal.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP1-44371-img0002.jpg "Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP1-44371] - Analysis of anti-LYPD3 antibody with human adrenal.")
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP1-44371] - Analysis of anti-LYPD3 antibody with skin, hair follicle.](http://images.novusbio.com/fullsize/C4.4A-LYPD3-Antibody-Immunohistochemistry-Paraffin-NBP1-44371-img0001.jpg)
![Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP3-14553] - C4.4A/LYPD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/C4.4A-LYPD3 Antibody-Immunohistochemistry-Paraffin-NBP3-14553-img0001.jpg "Immunohistochemistry-Paraffin: C4.4A/LYPD3 Antibody [NBP3-14553] - C4.4A/LYPD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Western Blot: C4.4A/LYPD3 Overexpression Lysate [NBP2-07341] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LYPD3.](http://images.novusbio.com/fullsize/LYPD3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07341-img0001.jpg "Western Blot: C4.4A/LYPD3 Overexpression Lysate [NBP2-07341] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LYPD3.")