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C4.4A/LYPD3 Antibody [Unconjugated]

 (3 Publications)
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Western blot shows lysates of SK‑BR‑3 human breast cancer cell line, MCF‑7 human breast cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for C4.4A/LYPD3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of SK‑BR‑3 human breast cancer cell line, MCF‑7 human breast cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5428-SP
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C4.4A/LYPD3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human C4.4A/LYPD3
Leu31-His286
Accession # O95274
Specificity
Detects human C4.4A/LYPD3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LYPD3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5428 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C4.4A/LYPD3 Antibody [Unconjugated]

  • C4.4A
  • C4.4A2310061G07Rik
  • GPI-anchored metastasis-associated protein C4.4A homolog
  • GPI-anchored metastasis-associated protein homolog
  • LY6/PLAUR domain containing 3
  • ly6/PLAUR domain-containing protein 3
  • LYPD3
  • Matrigel-induced gene C4 protein
  • MIG-C4

Background

LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI anchored metastasis-associated protein C4.4A) is an 85‑95 kDa, variably glycosylated member of the Ly6 family of GPI anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin‑3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296 amino acid (aa) GPI linked glycoprotein. It contains two UPAR domains
(aa 33‑126 and 140‑222), and an ADAM cleavage site (aa 312‑325). The molecule shows extensive N and O linked glycosylation. There is one potential isoform that shows a deletion of aa 129‑180. Over aa 31‑286, human LYPD3 shares 81% aa identity with mouse LYPD3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF5428
C4.4A/LYPD3 Antibody [Unconjugated]
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Publications for C4.4A/LYPD3 Antibody (AF5428)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol LYPD3
Uniprot