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BST2 Antibody - Azide Free

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Western Blot: BST2 Antibody - Azide Free [NBP2-45265] - Analysis using BST2 antibody. Human heart in the 1) absence and 2) presence of immunizing peptide, 3) human Jurkat, 4) mouse RAW lysate and 5) recombinant human ...read more
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - Analysis of BST2 in human liver tissue using BST2 antibody at 5 ug/mL. Image using the standard format of this product.
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - Mouse femur bone marrow section stained with BST2 antibody (red) and DAPI (blue). Image from a verified customer review. Image using the standard format of ...read more
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - BST-2 (green) was detected in human skin (nevus) using Ang-2 antibody (1:50; 1 hour) and secondary anti-rabbit-FITC (1:500; 30 min) in PBS. Nuclei were ...read more
Simple Western: BST2 Antibody - Azide Free [NBP2-45265] - Lane view shows a specific band for BST2 using 0.5 mg/mL Jurkat cell lysate and antibody at 1:200. Electoropherogram image of corresponding Simple Western lane ...read more
Simple Western: BST2 Antibody - Azide Free [NBP2-45265] - Simple Western lane view shows a specific band for BST2 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing A246 conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Pm, Pm-Or, RMSpecies Glossary
Applications WB, Simple Western, Flow, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide Free
Concentration
1.0 mg/ml

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BST2 Antibody - Azide Free Summary

Immunogen
A portion of amino acids 80-130 of human BST2 was used as the immunogen for this antibody.
Predicted Species
Orangutan (100%), Chimpanzee (100%), Rhesus Macaque (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BST2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (Crother et al (2011); PMID 21695198)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Simple Western 1:200
  • Western Blot 2-4 ug/ml
Application Notes
BST2 is a 180 amino acid protein with an observed molecular weight of approximately 30 kDa in western blotting (see Ishikawa et all, 1995 PMID 7607676). In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Reactivity Notes

The BST2 amino acid sequence used as immunogen for this antibody is 100% homologous in White-cheeked Gibbon, Great Ape, and Gibbon.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for BST2 Antibody - Azide Free

  • bone marrow stromal antigen 2
  • bone marrow stromal cell antigen 2
  • BST2
  • BST-2
  • CD317 antigen
  • CD317
  • HM1.24 Antigen
  • NPC-A-7
  • PDCA-1
  • Tetherin

Background

BST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, Nk cells and DCs (at protein level).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BST2