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BRCA1 Antibody (MU) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP
Clone
MU
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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BRCA1 Antibody (MU) [FITC] Summary

Immunogen
Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398]
Epitope
The epitope recognized is found between amino acids 1600-1749
Localization
nuclear
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BRCA1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for BRCA1 Antibody (MU) [FITC]

  • BRCA1
  • BRCAI
  • breast and ovarian cancer susceptibility protein 1
  • breast and ovarian cancer sususceptibility protein
  • breast cancer 1, early onset
  • breast cancer type 1 susceptibility protein
  • EC 6.3.2
  • EC 6.3.2.-
  • IRIS
  • PNCA4
  • PSCP
  • subunit 1

Background

BRCA1 (breast cancer early-onset 1) is a tumour-suppressor gene which was originally discovered on the basis of its linkage to early-onset breast or ovarian cancer in women. BRCA1 gene encodes a protein of 1863 amino acids with three distinct regions of protein interaction: RING domain, RAD51-interaction domain and BRCT (BRCA1 C-terminus) domain. The N-terminal RING finger domain binds to several proteins, including formation of heterodimers with BARD1 (BRCA1-associated RING domain protein). Both BRCA1 and BARD1 contain the RING finger domain which is important for tumour-suppression functions. The RAD51-interaction domain is enclosed by exon 11 and is involved in DNA-DSBs repair. It also contains multiple protein-binding sites, in addition to those for RAD51/RAD50 complex. Loss of RAD51 binding increase the risk of cancer as there could be an increment in the amount of damaged DNA. The C-terminus of BRCA1 contains an acidic domain that functions as a transcriptional activation domain and this domain contains a tandem repeat of approximately 95 amino acids called BRCT. This domain is also found in proteins such as BARD1 which is involved in cell-cycle control and DNA repair. BRCA1 has been implicated in diverse cancer-related activities, including roles in cell-cycle progression, DNA repair, DNA damage-responsive cell-cycle checkpoints, transcription regulation, ubiquitination, chromosome remodelling and apoptosis. Defects in BRCA1 are a cause of susceptibility to breast cancer, familial breast-ovarian cancer type 1 (BROVCA1), ovarian cancer, and pancreatic cancer type 4 (PNCA4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRCA1 Antibody (NB100-600F) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BRCA1 Antibody (NB100-600F) (0)

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Secondary Antibodies

 

Isotype Controls

Additional BRCA1 Products

Research Areas for BRCA1 Antibody (NB100-600F)

Find related products by research area.

Blogs on BRCA1. Showing 1-10 of 21 blog posts - Show all blog posts.

NPC1: A Potential Target For Triple-Negative Breast Cancer
By Natalia Gurule, PhD Breast Cancer is a Heterogeneous DiseaseBreast cancer is the most frequently identified malignancy in women, accounting for 30% of diagnosed cases of cancer in women in the US annuall...  Read full blog post.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily ...  Read full blog post.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels ...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage ...  Read full blog post.

BRCA1 - A Critical Tumor Suppressor Gene in Women
Breast cancer 1, early onset (BRCA1) is a well-known tumor suppressor gene that was originally discovered due to its link with early-onset breast and ovarian cancer in women. The BRCA1 protein contains the following domains: RING finger, RAD51-interac...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

Showing 1-10 of 21 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol BRCA1