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BMP-4 Antibody (3C11H8) - BSA Free

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Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against human BMP4 (AA: 277-408) recombinant protein. (Expected MW is 41 kDa).
Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis of Hela cells using BMP-4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 ...read more
Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mouse mAb against A549 (1) and C6 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11H8) [NBP2-52423] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 ...read more
ELISA: BMP-4 Antibody (3C11H8) [NBP2-52423] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, CyTOF-ready
Clone
3C11H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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BMP-4 Antibody (3C11H8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human BMP-4 (AA: 277-408) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BMP4
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BMP-4 Antibody (3C11H8) - BSA Free

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME

Background

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BMP4