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Recombinant Human BMP-3 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Human BMP-3 Protein Summary

Description
A homodimeric protein consisting of two 110 amino acid non-glycosylated polypeptide chains corresponding to BMP-3 Source: E. coli

Amino Acid Sequence: QWIEPRNCAR RYLKVDFADI GWSEWIISPK SFDAYYCSGA CQFPMPKSLK PSNHATIQSI VRAVGVVPGI PEPCCVPEKM SSLSILFFDE NKNVVLKVYP NMTVESCACR
Details of Functionality
BMP-3 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit BMP-2-induced activity in murine MC3T3-E1 cells.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
BMP3
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of BMP-3 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
24.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human BMP-3 Protein

  • BMP3
  • BMP-3
  • BMP3A
  • BMP-3A
  • bone morphogenetic protein 3 (osteogenic)
  • bone morphogenetic protein 3
  • Bone morphogenetic protein 3A
  • bone morphogenetic protein-3
  • Osteogenin

Background

Bone morphogenetic protein 3 (BMP3) is a 472 amino acid member of the bone morphogenetic protein (BMP) family. BMP3 negatively regulates bone density and is a member of the TGF beta superfamily. It is highly expressed in fracture tissue, predominantly in osteoblasts, osteoclasts and chondroblasts. It may play a role in otosclerosis, osteosarcoma, osteogenesis imperfecta, osteomalacia and periodontitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol BMP3