Reactivity | MuSpecies Glossary |
Applications | WB |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 1.0 mg/ml |
Immunogen | A fusion protein representing amino acids 171-356 of human BLNK. |
Specificity | The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65). |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | BLNK |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Predicted molecular weight 54.7 kDa. Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. |
Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C. Do not freeze. |
Buffer | PBS (pH 7.4) |
Preservative | 0.09% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BLNK Antibody (NB500-542)Find related products by research area.
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